Sun Zeng-feng, Wang Le, Gu Feng, Fu Li, Li Wen-liang, Ma Yong-jie
Department of Neurosurgery, Tianjin Medical University Cancer Institute & Hospital, Tianjin, China.
Zhonghua Zhong Liu Za Zhi. 2012 Jan;34(1):26-30.
To study the expression of Notch1, MMP-2 and MMP-9 in glioma patients and their relationship with progression and prognosis of gliomas.
Sixty-four cases of glioma were included in this study. There were four cases of grade 1 tumor, twenty-five cases of grade 2, nine cases of grade 3, and twenty-six cases of grade 4. Immunohistochemistry (SP staining method) was used to detect the expression of Notch1, MMP-2 and MMP-9 in glioma tissues and adjacent non-tumor tissues, and the patients were followed up.
Notch1, MMP-2 and MMP-9 were detected in glioma tissues but not in adjacent non-tumor tissues. The expression of Notch1 was increased with the pathological grade of the gliomas (r = 0.262, P < 0.05). The survival time of patients with strong expression of Notch1 was 31.0 months, significantly shorter than that of patients with non-strong positive (negative, weak and moderately) Notch1 expression (53.0 months, P < 0.05). Significant difference in survival time was observed between patients with negative and positive expression of MMP-9 (P < 0.05).
Notch1, MMP-2 and MMP-9 are closely correlated with the progression and prognosis of malignant gliomas. Notch1 may participate in the expression regulation of MMP-2 and MMP-9. Compared with MMP-2, MMP-9 may play a more important role in determining the prognosis of malignant glioma. Notch1 and MMP-9 may become new biological markers for prognosis of patients with malignant glioma.
研究Notch1、基质金属蛋白酶-2（MMP-2）和基质金属蛋白酶-9（MMP-9）在胶质瘤患者中的表达及其与胶质瘤进展和预后的关系。
本研究纳入64例胶质瘤患者。其中1级肿瘤4例，2级25例，3级9例，4级26例。采用免疫组织化学（SP染色法）检测胶质瘤组织及癌旁非肿瘤组织中Notch1、MMP-2和MMP-9的表达，并对患者进行随访。
在胶质瘤组织中检测到Notch1、MMP-2和MMP-9，而在癌旁非肿瘤组织中未检测到。Notch1的表达随胶质瘤病理分级增加而升高（r = 0.262，P < 0.05）。Notch1强表达患者的生存时间为31.0个月，显著短于Notch1非强阳性（阴性、弱阳性和中度阳性）表达的患者（53.0个月，P < 0.05）。MMP-9阴性和阳性表达患者的生存时间存在显著差异（P < 0.05）。
Notch1、MMP-2和MMP-9与恶性胶质瘤的进展和预后密切相关。Notch1可能参与MMP-2和MMP-9的表达调控。与MMP-2相比，MMP-9在决定恶性胶质瘤预后方面可能起更重要的作用。Notch1和MMP-9可能成为恶性胶质瘤患者预后的新生物标志物。
