[镁补充对大鼠血管钙化的影响]

Pen Ji-Xia, Li Li, Wang Xiong, Zhang Ya-Hui, Li Xue-Feng, Wu Sheng-Ying

Laboratory of Medical Functions, HuBei University of Medicine, the Affiliated Taihe Hospital, Shiyan 442000, China.

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2012 Jan;28(1):20-3.

OBJECTIVE

To observe the role of magnesium sulfate in vascular calcification, to explore the role and the mechanism of magnesium sulfate in vascular calcification.

METHODS

The vascular calcification model was established by administration of vitamin D3 plus nicotine (VDN) in SD rats. To estimate the extent of calcification by Von Kossa staining, calcium content and alkaline phosphatase activity, osteopontin (OPN) mRNA were determined by using semi-quantitative reverse-transcription polymerase chain reaction.The malondialdehyde (MDA) and nitric oxide (NO) content and activities of superoxide dismutase(SOD) were measured by biochemistry.

RESULTS

A strong positive staining of black/brown areas among the elastic fibers of the medial layer in calcified aorta by Von Kossa staining, calcium content and ALP activity in calcified arteries increased by 3.9-and 3.4-fold as compared with the controls. The expression of OPN mRNA was up-regulated by 40% (P < 0.01). The lipid peroxidation products MDA in vascular were increased 2.0-fold (P < 0.01). The NO content and SOD activity were greatly decreased by 64% and 72% (P < 0.01), respectively, compared with controls. However, calcium content and ALP activity in VDN plus magnesium sulfate group were lower than those in VDN group. Low and high dosage magnesium sulfate obviously relieved degree of calcification in the cardiovascular tissues in a dosage-dependent manner (P < 0.01).

CONCLUSION

Magnesium sulfate plays a role in the pathogenesis of vascular calcification by reducing vascular calcification and decreasing vascular injury.

目的

观察硫酸镁在血管钙化中的作用，探讨硫酸镁在血管钙化中的作用及机制。

方法

通过给SD大鼠注射维生素D3加尼古丁（VDN）建立血管钙化模型。采用Von Kossa染色法评估钙化程度，测定钙含量和碱性磷酸酶活性，用半定量逆转录聚合酶链反应检测骨桥蛋白（OPN）mRNA。采用生化方法测定丙二醛（MDA）、一氧化氮（NO）含量及超氧化物歧化酶（SOD）活性。

结果

Von Kossa染色显示钙化主动脉中层弹性纤维间有强阳性的黑/棕色区域，钙化动脉的钙含量和碱性磷酸酶活性较对照组分别增加3.9倍和3.4倍。OPN mRNA表达上调40%（P<0.01）。血管中脂质过氧化产物MDA增加2.0倍（P<0.01）。与对照组相比，NO含量和SOD活性分别显著降低64%和72%（P<0.01）。然而，VDN加硫酸镁组的钙含量和碱性磷酸酶活性低于VDN组。低剂量和高剂量硫酸镁均以剂量依赖方式明显减轻心血管组织的钙化程度（P<0.01）。