Harris L F, Hamparian V V, Young E A, Hughes J H
Cancer Res. 1979 Oct;39(10):3996-4002.
Cell-mediated immunity to fibroblasts transformed by herpes simplex virus type 2 was investigated with a lymphocyte assay system. The assay system was first standardized with phytohemagglutinin, a nonspecific stimulator of blastogenesis. Hamster splenic and blood lymphocytes reacted to phytohemagglutinin with a dose-response curve similar to that reported for other rodent species. Splenic lymphocytes from hamsters bearing isografts, induced by herpes simplex virus type 2, were transformed by cell-free virus-induced tumor antigens. The reactions with cell-free tumor antigens were dose-dependent and paralleled the findings with phytohemagglutinin. The initial transformation response of immune lymphocytes to homologous tumor antigens occurred after 72 hr incubation with antigen. Immune splenic lymphocytes from hamsters were also significantly stimulated with antigens obtained from cells productively infected with herpes simplex virus type 2. Immune lymphocytes were not stimulated with heterologous antigens from simian virus 40-transformed mouse or hamster cells. Likewise, lymphocytes from hamsters sensitized to cells transformed by simian virus 40 reacted with both simian virus 40-transformed mouse and hamster cells but did not react with cells transformed by a heterologous virus. The results suggest that under defined conditions a lymphocyte transformation assay may be useful for the specific detection of common viral-induced antigens on tumor cells.
利用淋巴细胞检测系统研究了对2型单纯疱疹病毒转化的成纤维细胞的细胞介导免疫。该检测系统首先用植物血凝素进行标准化，植物血凝素是一种非特异性的成淋巴细胞刺激剂。仓鼠脾淋巴细胞和血液淋巴细胞对植物血凝素的反应呈剂量反应曲线，与其他啮齿动物物种报道的相似。携带由2型单纯疱疹病毒诱导的同基因移植物的仓鼠的脾淋巴细胞，被无细胞病毒诱导的肿瘤抗原转化。与无细胞肿瘤抗原的反应是剂量依赖性的，与植物血凝素的结果相似。免疫淋巴细胞对同源肿瘤抗原的初始转化反应在与抗原孵育后72小时出现。来自仓鼠的免疫脾淋巴细胞也被从高效感染2型单纯疱疹病毒的细胞中获得的抗原显著刺激。免疫淋巴细胞未被来自猴病毒40转化的小鼠或仓鼠细胞的异源抗原刺激。同样，对猴病毒40转化的细胞致敏的仓鼠淋巴细胞与猴病毒40转化的小鼠和仓鼠细胞都有反应，但与异源病毒转化的细胞没有反应。结果表明，在特定条件下，淋巴细胞转化试验可能有助于特异性检测肿瘤细胞上常见的病毒诱导抗原。
