Jang H A, Cho S, Kang S G, Ko Y H, Kang S H, Bae J H, Cheon J, Kim J J, Lee J G
Department of Urology, Korea University Hospital, Seoul, Republic of Korea.
Urol Int. 2012;88(4):463-9. doi: 10.1159/000337206. Epub 2012 Apr 14.
To assess the effects of ginseng saponin on relaxation of the bladder and prostatic urethra and to determine its mechanism of action.
For the in vitro study, prostatic urethra muscle strips were harvested from 18 male New Zealand rabbits. The strips were mounted in organ baths and connected to force displacement transducers. After stabilization, maximal tissue contractions were obtained by the application of phenylepinephrine to the urethra strips, and a dose-response curve for ginseng saponin was constructed (10(-6)-10(-2)M). After pretreatment of urethra strips with N-nitro-L-arginine methyl ester (L-NAME), another dose-response curve for ginseng saponin was constructed. For the in vivo study, we used adult male Sprague-Dawley rats divided into three groups [control, partial bladder outlet obstruction (PBOO) and saponin-fed groups], and we monitored the vesical pressure (P(ves)) and urethral perfusion pressure (UPP).
The ginseng saponin induced a significant dose-dependent relaxant effect on the prostatic urethra strips. A significant relaxant effect of ginseng saponin was observed from 10(-3)M, and ginseng saponin significantly relaxed urethra strips by 50.2 ± 20.26% at 10(-2)M. The relaxant effect was partially inhibited with L-NAME pretreatment. In the in vivo study, the change in UPP between baseline and relaxation was significantly higher in the saponin group than in the control or PBOO group (p < 0.001). The saponin group showed a significantly lower baseline P(ves) than the PBOO group.
We observed a significant relaxation effect of ginseng saponin on the bladder and prostatic urethra in both in vitro and in vivo studies. The mechanism by which ginseng saponin induces relaxation appears to involve the nitric oxide/nitric oxide synthase pathway.
评估人参皂苷对膀胱和前列腺尿道舒张的作用，并确定其作用机制。
在体外研究中，从18只雄性新西兰兔获取前列腺尿道肌条。将肌条安装在器官浴槽中并连接到力位移换能器。稳定后，通过向尿道肌条施加去氧肾上腺素获得最大组织收缩，并构建人参皂苷的剂量 - 反应曲线（10⁻⁶ - 10⁻²M）。用N - 硝基 - L - 精氨酸甲酯（L - NAME）预处理尿道肌条后，再构建一条人参皂苷的剂量 - 反应曲线。在体内研究中，我们使用成年雄性Sprague - Dawley大鼠，分为三组[对照组、部分膀胱出口梗阻（PBOO）组和皂苷喂养组]，并监测膀胱压力（P(ves)）和尿道灌注压力（UPP）。
人参皂苷对前列腺尿道肌条有显著的剂量依赖性舒张作用。从10⁻³M开始观察到人参皂苷有显著的舒张作用，在10⁻²M时人参皂苷使尿道肌条显著舒张50.2 ± 20.26%。L - NAME预处理后，舒张作用部分受到抑制。在体内研究中，皂苷组基线与舒张之间UPP的变化显著高于对照组或PBOO组（p < 0.001）。皂苷组的基线P(ves)显著低于PBOO组。
我们在体外和体内研究中均观察到人参皂苷对膀胱和前列腺尿道有显著的舒张作用。人参皂苷诱导舒张的机制似乎涉及一氧化氮/一氧化氮合酶途径。
