Center for Anatomical Science and Education, Saint Louis University, St. Louis, MO, USA.
J Orthop Res. 2012 Oct;30(10):1604-10. doi: 10.1002/jor.22124. Epub 2012 Apr 16.
The goal of this study was to develop an aggressive running regimen for modeling osteoarthritis (OA) in rats. Twelve Wistar rats were randomly placed into either a running group or a non-running group to serve as the control. The running rats used a motorized treadmill to run either 30 km in 3 weeks or 55 km in 6 weeks. Each week, the prints of hind paws were obtained when rats were made to walk through a tunnel. The resulting prints were digitalized for analyses of stride length and step angle. The histology of the knees was examined at 3 and 6 weeks and the OA pathology in the knees was quantified by Mankin's score. Osteoarthritic pathology developed in the knees of the running rats, including decreased proteoglycan content, uneven type II collagen distribution in the cartilage matrix, increased MMP-13 expression, expanded calcified cartilage zone, and clefts and defects in articular cartilage. The pathology worsened from running for 3 to 6 weeks. Gait analysis revealed an inverse correlation between paw angle and the grades of OA pathology. In conclusion, excessive running induces joint degeneration and a unique gait pattern in rats.
本研究旨在制定一种激进的跑步方案，以在大鼠中建立骨关节炎（OA）模型。将 12 只 Wistar 大鼠随机分为跑步组或非跑步组作为对照组。跑步大鼠使用电动跑步机在 3 周内跑 30 公里或在 6 周内跑 55 公里。每周，当大鼠通过隧道行走时，都会获取后爪的印痕。对印痕进行数字化处理，以分析步幅长度和步幅角度。在 3 周和 6 周时检查膝关节的组织学，并通过 Mankin 评分对膝关节的 OA 病理进行定量分析。跑步大鼠的膝关节出现了骨关节炎病理，包括：软骨基质中蛋白聚糖含量减少、II 型胶原分布不均匀、MMP-13 表达增加、钙化软骨区扩大以及关节软骨出现裂隙和缺损。跑步 3 到 6 周后，病理恶化。步态分析显示，爪角与 OA 病理分级呈负相关。总之，过度跑步会导致大鼠关节退化和独特的步态模式。
