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分析维生素 C 缺乏时 gulo(-/-) 小鼠各器官中维生素 C 浓度。

The analysis of vitamin C concentration in organs of gulo(-/-) mice upon vitamin C withdrawal.

机构信息

Department of Anatomy, Medical Research Center, Seoul National University, Seoul 110-799, Korea.

出版信息

Immune Netw. 2012 Feb;12(1):18-26. doi: 10.4110/in.2012.12.1.18. Epub 2012 Feb 29.

DOI:10.4110/in.2012.12.1.18
PMID:22536166
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3329599/
Abstract

BACKGROUND

Vitamin C is an essential nutrient for maintaining human life. Vitamin C insufficiency in the plasma is closely related with the development of scurvy. However, in vivo kinetics of vitamin C regarding its storage and consumption is still largely unknown.

METHODS

We used Gulo(-/-) mice, which cannot synthesize vitamin C like human. Vitamin C level in plasma and organs from Gulo(-/-) mice was examined, and it compared with the level of wild-type mice during 5 weeks.

RESULTS

The significant weight loss of Gulo(-/-) mice was shown at 3 weeks after vitamin C withdrawal. However, there was no differences between wild-type and vitamin C-supplemented Gulo(-/-) mice (3.3 g/L in drinking water). The concentration of vitamin C in plasma and organs was significantly decreased at 1 week after vitamin C withdrawal. Vitamin C is preferentially deposited in adrenal gland, lymph node, lung, and brain. There were no significant changes in the numbers and CD4/CD8 ratio of splenocytes in Gulo(-/-) mice with vitamin C withdrawal for 4 weeks. And the architecture of spleen in Gulo(-/-) mice was disrupted at 5 weeks after vitamin C withdrawal.

CONCLUSION

The vitamin C level of Gulo(-/-) mice was considerably decreased from 1 week after vitamin C withdrawal. Vitamin C is preferentially stored in some organs such as brain, adrenal gland and lung.

摘要

背景

维生素 C 是维持人类生命所必需的营养物质。血浆中维生素 C 不足与坏血病的发展密切相关。然而，维生素 C 在体内的储存和消耗动力学仍知之甚少。

方法

我们使用了类似于人类无法合成维生素 C 的 Gulo(-/-) 小鼠。检查了 Gulo(-/-) 小鼠血浆和器官中的维生素 C 水平，并在 5 周内与野生型小鼠的水平进行了比较。

结果

Gulo(-/-) 小鼠在停止补充维生素 C 后 3 周体重明显减轻。然而，在补充维生素 C 的野生型和 Gulo(-/-) 小鼠之间没有差异（饮用水中 3.3g/L）。停止补充维生素 C 1 周后，血浆和器官中的维生素 C 浓度明显下降。维生素 C 优先沉积在肾上腺、淋巴结、肺和脑。停止补充维生素 C 4 周后，Gulo(-/-) 小鼠的脾细胞数量和 CD4/CD8 比值没有明显变化。停止补充维生素 C 5 周后，Gulo(-/-) 小鼠的脾脏结构被破坏。

结论

Gulo(-/-) 小鼠的维生素 C 水平在停止补充维生素 C 后 1 周内显著下降。维生素 C 优先储存在某些器官，如脑、肾上腺和肺。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aaa/3329599/b01a5f74343a/in-12-18-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aaa/3329599/2d35487022df/in-12-18-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aaa/3329599/e780836afb58/in-12-18-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aaa/3329599/e69b31596115/in-12-18-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aaa/3329599/65c1214b0cc7/in-12-18-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aaa/3329599/b01a5f74343a/in-12-18-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aaa/3329599/2d35487022df/in-12-18-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aaa/3329599/e780836afb58/in-12-18-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aaa/3329599/e69b31596115/in-12-18-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aaa/3329599/65c1214b0cc7/in-12-18-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6aaa/3329599/b01a5f74343a/in-12-18-g005.jpg

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