Department of Biological Science, Graduate School of Science and Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.
J Fish Biol. 2012 May;80(6):2236-52. doi: 10.1111/j.1095-8649.2012.03277.x. Epub 2012 Apr 10.
The structure and immunolocalization of the ion transporters Na(+) ,K(+) -ATPase (NKA), Na(+) /H(+) exchanger (NHE3) and vacuolar-type H(+) -ATPase (VHA) were examined in the gills of teleosts of the family Blenniidae, which inhabit rocky shores with vertical zonation in subtropical seas. These features were compared among the following species with different ecologies: the amphibious rockskipper blenny Andamia tetradactylus, the intertidal white-finned blenny Praealticus tanegasimae and the purely marine yaeyama blenny Ecsenius yaeyamaensis. Light and electron microscopic observations indicated that thick gill filaments were arranged close to each other and alternately on two hemibranches of a gill arch in the opercular space of A. tetradactylus. Many mucous cells (MC) and mitochondrion-rich cells (MRC) were present in the interlamellar regions of the gill filament. An immunohistochemical study demonstrated that numerous NKA, NHE3 and some VHA were located predominantly on presumed MRCs of gill filaments and at the base of the lamellae. Analyses using serial (mirror image) sections of the gills indicated that only a few NKA immunoreactive cells (IRC) were colocalized with VHA on some MRCs in the filaments. In the gills of P. tanegasimae, NKA- and NHE3-IRCs were observed in the interlamellar region of the filaments and at the base of the lamellae. VHA-IRCs were located sparsely on the lamellae and filaments. In the gills of E. yaeyamaensis, the lamellae and filaments were thin and straight, respectively. MCs were located at the tip as well as found scattered in the interlamellar region of gill filaments. NKA-, NHE3- and VHA-IRCs were moderately frequently observed in the filaments and rarely on the lamellae. This study shows that the structure and distribution of ion transporters in the gills differ among the three blennid species, presumably reflecting their different ecologies.
本文研究了栖息于亚热带海域具有垂直分区的滨螺科鱼类的鳃中离子转运体 Na(+),K(+) -ATPase (NKA)、Na(+) /H(+) 交换器 (NHE3) 和液泡型 H(+) -ATPase (VHA)的结构和免疫定位。将这三种不同生态的鱼类的离子转运体结构和分布进行了比较：具有两栖生活习性的岩鱚、潮间带生活的白鳍滨螺和纯海洋生活的雅氏鱚。光镜和电镜观察表明，厚的鳃丝在掩蔽空间的鳃弓两个半鳃上彼此靠近且交替排列。许多黏液细胞 (MC) 和富含线粒体的细胞 (MRC) 存在于鳃丝的片层间区。免疫组织化学研究表明，大量的 NKA、NHE3 和一些 VHA 主要位于鳃丝的假定 MRC 上和片层的基部。对鳃的连续 (镜像) 切片进行分析表明，在一些 MRC 上，只有少数 NKA 免疫反应细胞 (IRC) 与 VHA 共定位。在 P. tanegasimae 的鳃中，NKA 和 NHE3-IRC 存在于鳃丝的片层间区和片层的基部。VHA-IRC 稀疏地位于片层和鳃丝上。在 E. yaeyamaensis 的鳃中，片层和鳃丝分别很薄且直。MC 位于鳃丝的尖端，也存在于鳃丝的片层间区。在鳃丝中中等频率地观察到 NKA、NHE3 和 VHA-IRC，在片层上很少观察到。本研究表明，三种滨螺科鱼类的鳃中离子转运体的结构和分布存在差异，这可能反映了它们不同的生态。
