X射线照射下金纳米管基质中超螺旋DNA单链断裂增强。
Enhanced single strand breaks of supercoiled DNA in a matrix of gold nanotubes under X-ray irradiation.
作者信息
Carter Joshua D, Cheng Neal N, Qu Yongquan, Suarez George D, Guo Ting
机构信息
Department of Chemistry, University of California, Davis, CA 95616, USA.
出版信息
J Colloid Interface Sci. 2012 Jul 15;378(1):70-6. doi: 10.1016/j.jcis.2012.04.003. Epub 2012 Apr 11.
Single-strand-breaks (SSBs) of supercoiled DNA (scDNA) molecules were used to probe the enhancement of X-ray radiation effect on scDNA mixed with gold nanotubes (AuNTs) in water. The amounts of measured enhancements using SSBs were significantly lower than the expected increase in energy deposition in water by AuNTs under hard X-ray irradiation. Three factors were identified to negatively affect the enhancement: (1) Attenuation of kinetic energies carried by electrons escaped from AuNTs, (2) Scavenging of OH radicals (˙OH) by the surface of bare AuNTs, and (3) Steric effect due to soluble scDNA molecules away from the surface of AuNTs. Benefits and limits of using gold nanomaterials as radiation enhancers and contrast agents are discussed.
利用超螺旋DNA（scDNA）分子的单链断裂（SSB）来探测水中与金纳米管（AuNTs）混合的scDNA上X射线辐射效应的增强情况。在硬X射线照射下，使用SSB测量的增强量显著低于AuNTs在水中预期的能量沉积增加量。确定了三个对增强有负面影响的因素：（1）从AuNTs逸出的电子携带的动能衰减；（2）裸露的AuNTs表面对OH自由基（˙OH）的清除；（3）可溶性scDNA分子远离AuNTs表面产生的空间位阻效应。讨论了使用金纳米材料作为辐射增强剂和造影剂的益处与局限性。
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