Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.
Clin Biochem. 2012 Jul;45(10-11):827-34. doi: 10.1016/j.clinbiochem.2012.04.004. Epub 2012 Apr 19.
To investigate the association of expression status of α-enolase (ENO1) and clinicopathological outcomes of CCA patients.
Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to compare differential expressed protein profiles of four human CCA cell lines and H69, a non-malignant biliary cell line, as a control. Immunohistochemical analysis was carried out in tissue-microarray of human CCA tissues (n=301).
We identified ENO1 in all CCA cell lines but not H69 by proteomics based. About 75% of patients with CCA showed over-expression of ENO1 in hyperplastic bile duct and the tumors compared with that in tumor-adjacent normal tissue counterparts. Moreover, over-expression of ENO1 is significantly associated with poor prognosis and tumor invasion of CCA patients.
ENO1 may serve as a prognostic marker to monitor the disease progression of these patients.
探讨烯醇化酶(ENO1)的表达状态与 CCA 患者临床病理结局的关系。
采用二维凝胶电泳(2-DE)和质谱(MS)技术比较了人 CCA 细胞系和 H69(一种非恶性胆管细胞系)作为对照的差异表达蛋白图谱。对人 CCA 组织的组织微阵列(n=301)进行免疫组织化学分析。
通过基于蛋白质组学的方法,我们在所有 CCA 细胞系中均鉴定到 ENO1,但在 H69 中未鉴定到。与肿瘤旁正常组织相比,约 75%的 CCA 患者的增生性胆管和肿瘤中 ENO1 呈过度表达。此外,ENO1 的过度表达与 CCA 患者的不良预后和肿瘤侵袭显著相关。
ENO1 可作为监测这些患者疾病进展的预后标志物。