• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人巨细胞病毒 pUL69 的 UAP56 相互作用基序转移到其鼠巨细胞病毒同源物中，将该蛋白转化为功能性 mRNA 输出因子，可在病毒感染期间替代 pUL69。

Transfer of the UAP56 interaction motif of human cytomegalovirus pUL69 to its murine cytomegalovirus homolog converts the protein into a functional mRNA export factor that can substitute for pUL69 during viral infection.

机构信息

Institute for Clinical and Molecular Virology, University of Erlangen-Nuremberg, Erlangen, Germany.

出版信息

J Virol. 2012 Jul;86(13):7448-53. doi: 10.1128/JVI.00730-12. Epub 2012 May 2.

DOI:10.1128/JVI.00730-12
PMID:22553320
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3416354/
Abstract

Nucleocytoplasmic shuttling and interaction with the cellular mRNA export factor UAP56 are prerequisites for the mRNA export activity of human cytomegalovirus (HCMV) pUL69. Although the murine cytomegalovirus homolog pM69 shuttles, it fails to export mRNAs due to its inability to recruit UAP56. However, chimeric proteins comprising pM69 fused to N-terminal pUL69 fragments, including its UAP56 interaction motif, acquire mRNA export activity. Importantly, growth curves of recombinant HCMVs illustrate that such a chimeric protein, but not pM69, substitutes for pUL69 during HCMV infection.

摘要

核质穿梭和与细胞 mRNA 输出因子 UAP56 的相互作用是人类巨细胞病毒 (HCMV) pUL69 的 mRNA 输出活性的前提条件。尽管鼠巨细胞病毒同源物 pM69 穿梭，但由于无法招募 UAP56，它无法输出 mRNAs。然而，包含 pM69 与 N 端 pUL69 片段融合的嵌合蛋白，包括其 UAP56 相互作用基序，获得了 mRNA 输出活性。重要的是，重组 HCMV 的生长曲线表明，在 HCMV 感染期间，这种嵌合蛋白而不是 pM69 替代 pUL69。

相似文献

1
Transfer of the UAP56 interaction motif of human cytomegalovirus pUL69 to its murine cytomegalovirus homolog converts the protein into a functional mRNA export factor that can substitute for pUL69 during viral infection.人巨细胞病毒 pUL69 的 UAP56 相互作用基序转移到其鼠巨细胞病毒同源物中，将该蛋白转化为功能性 mRNA 输出因子，可在病毒感染期间替代 pUL69。
J Virol. 2012 Jul;86(13):7448-53. doi: 10.1128/JVI.00730-12. Epub 2012 May 2.
2
Characterization of the betaherpesviral pUL69 protein family reveals binding of the cellular mRNA export factor UAP56 as a prerequisite for stimulation of nuclear mRNA export and for efficient viral replication.β疱疹病毒 pUL69 蛋白家族的特征表明，与细胞 mRNA 输出因子 UAP56 的结合是刺激核 mRNA 输出和有效病毒复制的前提条件。
J Virol. 2011 Feb;85(4):1804-19. doi: 10.1128/JVI.01347-10. Epub 2010 Dec 8.
3
pUL69 of Human Cytomegalovirus Recruits the Cellular Protein Arginine Methyltransferase 6 via a Domain That Is Crucial for mRNA Export and Efficient Viral Replication.人巨细胞病毒的pUL69通过一个对mRNA输出和高效病毒复制至关重要的结构域招募细胞蛋白精氨酸甲基转移酶6。
J Virol. 2015 Sep;89(18):9601-15. doi: 10.1128/JVI.01399-15. Epub 2015 Jul 15.
4
Phosphosite Analysis of the Cytomegaloviral mRNA Export Factor pUL69 Reveals Serines with Critical Importance for Recruitment of Cellular Proteins Pin1 and UAP56/URH49.巨细胞病毒 mRNA 输出因子 pUL69 的磷酸化位点分析揭示了丝氨酸对招募细胞蛋白 Pin1 和 UAP56/URH49 的关键重要性。
J Virol. 2020 Mar 31;94(8). doi: 10.1128/JVI.02151-19.
5
The UL69 transactivator protein of human cytomegalovirus interacts with DEXD/H-Box RNA helicase UAP56 to promote cytoplasmic accumulation of unspliced RNA.人巨细胞病毒的UL69反式激活蛋白与DEXD/H盒RNA解旋酶UAP56相互作用，以促进未剪接RNA的细胞质积累。
Mol Cell Biol. 2006 Mar;26(5):1631-43. doi: 10.1128/MCB.26.5.1631-1643.2006.
6
Binding of the human cytomegalovirus (HCMV) tegument protein UL69 to UAP56/URH49 is not required for efficient replication of HCMV.人巨细胞病毒（HCMV）的衣壳蛋白 UL69 与 UAP56/URH49 的结合对于 HCMV 的有效复制并非必需。
J Virol. 2010 Sep;84(18):9649-54. doi: 10.1128/JVI.00669-10. Epub 2010 Jul 7.
7
RNA-binding of the human cytomegalovirus transactivator protein UL69, mediated by arginine-rich motifs, is not required for nuclear export of unspliced RNA.由富含精氨酸的基序介导的人巨细胞病毒反式激活蛋白UL69的RNA结合对于未剪接RNA的核输出并非必需。
Nucleic Acids Res. 2006 Feb 25;34(4):1237-49. doi: 10.1093/nar/gkl007. Print 2006.
8
Cyclin-dependent Kinases Phosphorylate the Cytomegalovirus RNA Export Protein pUL69 and Modulate Its Nuclear Localization and Activity.细胞周期蛋白依赖性激酶使巨细胞病毒RNA输出蛋白pUL69磷酸化，并调节其核定位和活性。
J Biol Chem. 2009 Mar 27;284(13):8605-13. doi: 10.1074/jbc.M805693200. Epub 2009 Jan 29.
9
Interactions of human cytomegalovirus proteins with the nuclear transport machinery.人类巨细胞病毒蛋白与核转运机制的相互作用。
Curr Top Microbiol Immunol. 2008;325:167-85. doi: 10.1007/978-3-540-77349-8_10.
10
Recruitment of cyclin-dependent kinase 9 to nuclear compartments during cytomegalovirus late replication: importance of an interaction between viral pUL69 and cyclin T1.巨细胞病毒晚期复制过程中环依赖激酶 9 向核区的募集：病毒 pUL69 和细胞周期蛋白 T1 之间相互作用的重要性。
J Gen Virol. 2011 Jul;92(Pt 7):1519-1531. doi: 10.1099/vir.0.030494-0. Epub 2011 Mar 30.

引用本文的文献

1
Role of human herpesvirus homologs of infected cell protein 27 (ICP27) in the biogenesis, processing, and maturation of mRNAs.感染细胞蛋白27（ICP27）的人类疱疹病毒同源物在mRNA生物合成、加工和成熟中的作用。
mBio. 2025 Apr 9;16(4):e0029125. doi: 10.1128/mbio.00291-25. Epub 2025 Mar 4.
2
Phosphosite Analysis of the Cytomegaloviral mRNA Export Factor pUL69 Reveals Serines with Critical Importance for Recruitment of Cellular Proteins Pin1 and UAP56/URH49.巨细胞病毒 mRNA 输出因子 pUL69 的磷酸化位点分析揭示了丝氨酸对招募细胞蛋白 Pin1 和 UAP56/URH49 的关键重要性。
J Virol. 2020 Mar 31;94(8). doi: 10.1128/JVI.02151-19.
3
The ICP27 Homology Domain of the Human Cytomegalovirus Protein UL69 Adopts a Dimer-of-Dimers Structure.人巨细胞病毒蛋白 UL69 的 ICP27 同源结构域采用二聚体-二聚体结构。
mBio. 2018 Jun 19;9(3):e01112-18. doi: 10.1128/mBio.01112-18.
4
Complete Genome Sequence of a Human Cytomegalovirus Strain AD169 Bacterial Artificial Chromosome Clone.人巨细胞病毒AD169株细菌人工染色体克隆的全基因组序列
Genome Announc. 2016 Mar 31;4(2):e00091-16. doi: 10.1128/genomeA.00091-16.
5
The eIF4AIII RNA helicase is a critical determinant of human cytomegalovirus replication.真核翻译起始因子4AIII（eIF4AIII）RNA解旋酶是人类巨细胞病毒复制的关键决定因素。
Virology. 2016 Feb;489:194-201. doi: 10.1016/j.virol.2015.12.009. Epub 2016 Jan 8.
6
pUL69 of Human Cytomegalovirus Recruits the Cellular Protein Arginine Methyltransferase 6 via a Domain That Is Crucial for mRNA Export and Efficient Viral Replication.人巨细胞病毒的pUL69通过一个对mRNA输出和高效病毒复制至关重要的结构域招募细胞蛋白精氨酸甲基转移酶6。
J Virol. 2015 Sep;89(18):9601-15. doi: 10.1128/JVI.01399-15. Epub 2015 Jul 15.

本文引用的文献

1
Characterization of the betaherpesviral pUL69 protein family reveals binding of the cellular mRNA export factor UAP56 as a prerequisite for stimulation of nuclear mRNA export and for efficient viral replication.β疱疹病毒 pUL69 蛋白家族的特征表明，与细胞 mRNA 输出因子 UAP56 的结合是刺激核 mRNA 输出和有效病毒复制的前提条件。
J Virol. 2011 Feb;85(4):1804-19. doi: 10.1128/JVI.01347-10. Epub 2010 Dec 8.
2
Binding of the human cytomegalovirus (HCMV) tegument protein UL69 to UAP56/URH49 is not required for efficient replication of HCMV.人巨细胞病毒（HCMV）的衣壳蛋白 UL69 与 UAP56/URH49 的结合对于 HCMV 的有效复制并非必需。
J Virol. 2010 Sep;84(18):9649-54. doi: 10.1128/JVI.00669-10. Epub 2010 Jul 7.
3
The many roles of the regulatory protein ICP27 during herpes simplex virus infection.调节蛋白ICP27在单纯疱疹病毒感染过程中的多种作用。
Front Biosci. 2008 May 1;13:5241-56. doi: 10.2741/3078.
4
The human cytomegalovirus regulatory protein UL69 and its effect on mRNA export.人类巨细胞病毒调节蛋白UL69及其对mRNA输出的影响。
Front Biosci. 2008 Jan 1;13:2939-49. doi: 10.2741/2899.
5
Multimerization of human cytomegalovirus regulatory protein UL69 via a domain that is conserved within its herpesvirus homologues.人巨细胞病毒调节蛋白UL69通过其疱疹病毒同源物中保守的结构域进行多聚化。
J Gen Virol. 2007 Feb;88(Pt 2):405-410. doi: 10.1099/vir.0.82480-0.
6
Deletion of open reading frame UL26 from the human cytomegalovirus genome results in reduced viral growth, which involves impaired stability of viral particles.从人巨细胞病毒基因组中删除开放阅读框UL26会导致病毒生长减缓，这涉及病毒颗粒稳定性受损。
J Virol. 2006 Jun;80(11):5423-34. doi: 10.1128/JVI.02585-05.
7
Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli.用于大肠杆菌中通用高效无标记DNA操作的两步红色介导重组。
Biotechniques. 2006 Feb;40(2):191-7. doi: 10.2144/000112096.
8
The UL69 transactivator protein of human cytomegalovirus interacts with DEXD/H-Box RNA helicase UAP56 to promote cytoplasmic accumulation of unspliced RNA.人巨细胞病毒的UL69反式激活蛋白与DEXD/H盒RNA解旋酶UAP56相互作用，以促进未剪接RNA的细胞质积累。
Mol Cell Biol. 2006 Mar;26(5):1631-43. doi: 10.1128/MCB.26.5.1631-1643.2006.
9
Assaying nuclear messenger RNA export in human cells.检测人类细胞中的细胞核信使核糖核酸输出
Methods Mol Biol. 2004;257:85-92. doi: 10.1385/1-59259-750-5:085.
10
A novel nuclear export signal and a REF interaction domain both promote mRNA export by the Epstein-Barr virus EB2 protein.一种新型核输出信号和一个REF相互作用结构域均通过爱泼斯坦-巴尔病毒EB2蛋白促进mRNA输出。
J Biol Chem. 2003 Jan 3;278(1):335-42. doi: 10.1074/jbc.M208656200. Epub 2002 Oct 25.