内皮素-1对体外培养的人小梁网细胞细胞骨架蛋白F-肌动蛋白的影响。
Effects of endothelin-1 on the cytoskeleton protein F-actin of human trabecular meshwork cells in vitro.
作者信息
Xiang Yan, Li Bin, Li Gui-Gang, Wang Rui-Lin, Chen Zhi-Qi, Xu Ling-Juan, Chen Lan, Shi Hui, Zhang Hong
机构信息
Department of Ophthalmology, Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.
出版信息
Int J Ophthalmol. 2010;3(1):61-3. doi: 10.3980/j.issn.2222-3959.2010.01.14. Epub 2010 Mar 18.
AIM
To observe the effect of endothelin-1 (ET-1) on the cytoskeleton protein F-actin of cultured human trabecular meshwork (HTM) cells.
METHODS
CULTURED HTM CELLS WERE RANDOMLY DIVIDED INTO FOUR GROUPS: control group, low-dose ET-1 (10(-9) mol/L) treatment group, middle-dose ET-1 (10(-8) mol/L) treatment group, and high-dose ET-1(10(-7) mol/L) treatment group. After treated with ET-1, the expression of cytoskeleton protein F-actin in trabecular meshwork was analyzed with Western-blot and the distribution of F-actin was detected with FITC-Phalloidin probe.
RESULTS
ET-1 dose-dependently and significantly increased F-actin in trabecular meshwork cells (P<0.05). The F-actin stress fiber and periphery actin fiber highly increased and manifested mild reorganization after treated with ET-1; and there were much more cell-to-cell and cell-to-extracellular matrix attachments formation in ET-1 treated HTM cells than that in the untreated HTM cells.
CONCLUSION
ET-1 promotes the expression of cytoske- leton protein F-actin and induced the trabecular meshwork actin cytoskeleton reorganization.
目的
观察内皮素-1（ET-1）对培养的人小梁网（HTM）细胞骨架蛋白F-肌动蛋白的影响。
方法
将培养的HTM细胞随机分为四组：对照组、低剂量ET-1（10⁻⁹ mol/L）处理组、中剂量ET-1（10⁻⁸ mol/L）处理组和高剂量ET-1（10⁻⁷ mol/L）处理组。用ET-1处理后，采用蛋白质免疫印迹法分析小梁网中细胞骨架蛋白F-肌动蛋白的表达，并用异硫氰酸荧光素-鬼笔环肽探针检测F-肌动蛋白的分布。
结果
ET-1剂量依赖性地显著增加小梁网细胞中的F-肌动蛋白（P<0.05）。用ET-1处理后，F-肌动蛋白应力纤维和周边肌动蛋白纤维高度增加并表现出轻度重组；与未处理的HTM细胞相比，ET-1处理的HTM细胞中细胞间和细胞与细胞外基质的附着形成更多。
结论
ET-1促进细胞骨架蛋白F-肌动蛋白的表达并诱导小梁网肌动蛋白细胞骨架重组。
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