Uppsala BioCenter, Department of Forest Mycology & Pathology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden.
Fungal Biol. 2012 May;116(5):620-9. doi: 10.1016/j.funbio.2012.03.001. Epub 2012 Mar 16.
Phlebiopsis gigantea has been widely used as the biocontrol fungus against the root and butt rot disease of conifers caused by Heterobasidion annosum. We investigated the regulation of two hydrophobin genes (Pgh1 and Pgh2) in strong and weak antagonistic isolates of the biological control agent P. gigantea under diverse substrate conditions. Transcript abundance of Pgh1 was higher in single cultures of strong performing isolates than in the weak performing isolates at the early and late stages of the fungal growth (P =0.05). Higher fold transcript changes of Pgh1 and Pgh2 were observed in the strong performing isolates at the early stage of the antagonistic interaction on modified Norkrans sawdust agar medium compared to the weak performing isolates. Higher transcript abundance of the two genes was also observed during growth in submerged compared to surface agar cultures (P<0.003 and P=0.0001 for Pgh1 and Pgh2, respectively). No correlation between antagonistic ability and sequence characteristics of either gene was found but a significant correlation was found between some strong performing isolates and the expression of Pgh1. Regulatory patterns of both Pgh1 and Pgh2 suggest a role during early stages of interaction between the two fungi and their potential roles in the biological control process is discussed.
巨鳞白腐菌被广泛用作防治由密环菌引起的针叶树根腐和干腐病的生防真菌。我们研究了不同基质条件下，生物防治剂巨鳞白腐菌强拮抗和弱拮抗分离株中两个疏水蛋白基因（Pgh1 和 Pgh2）的调控。在真菌生长的早期和晚期，强表现分离株的 Pgh1 转录丰度高于弱表现分离株（P =0.05）。与弱表现分离株相比，在改良的 Norkrans 锯末琼脂培养基上拮抗相互作用的早期阶段，强表现分离株中 Pgh1 和 Pgh2 的转录倍数变化更高。与表面琼脂培养相比，在浸没培养中观察到这两个基因的转录丰度更高（P<0.003 和 P=0.0001 分别用于 Pgh1 和 Pgh2）。没有发现拮抗能力与这两个基因的序列特征之间的相关性，但在一些强表现分离株和 Pgh1 的表达之间发现了显著的相关性。Pgh1 和 Pgh2 的调控模式表明它们在两种真菌相互作用的早期阶段发挥作用，并讨论了它们在生物防治过程中的潜在作用。
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