UFR of Management and Environmental Sciences, University of Abobo Adjame, 02 bp 801, Abijan 02, Cote d'Ivoire.
Bull Environ Contam Toxicol. 2012 Jul;89(1):44-52. doi: 10.1007/s00128-012-0617-8. Epub 2012 May 6.
Immunohistological and histopathological methods were used to highlight the importance of cell damages and some biomarkers for health risk assessment. A comparative study between 3 sites of the lagoons showed that the most polluted areas (Adiake and Ebrah), influenced by human activities, presented more damaged cells and stained cells in gills and livers of Chrysichthys nigrodigitatus than the less polluted area (Layo): gill cell hyperplasia and liver cells vacuolation were more evident in fish from Adiake than in fish from Ebrah and Layo. The percentage of proliferating cell nuclear antigen (PCNA)-positive cells in gill were 45.8 % ± 23.7 % for Adiake, 18.2 % ± 4.6 % for Ebrah and 11.4 % ± 6.51 % for Layo; The percentage of PCNA-positive in liver cells were 3.8 % ± 3.6 % for Adiake, 4.9 % ± 4.7 % for Ebrah and 2.6 % ± 2.5 % for Layo. Gills were more affected than livers. The Adiake site was the most contaminated area of the lagoon complex of Cote d'Ivoire.
采用免疫组织化学和组织病理学方法强调了细胞损伤和一些生物标志物对健康风险评估的重要性。对泻湖 3 个地点进行的对比研究表明，受人类活动影响污染最严重的地区（阿迪亚克和埃布拉），其鳃和鱼肝中受损细胞和染色细胞比污染较轻的拉约地区更多：阿迪亚克的鱼比埃布拉和拉约的鱼更明显出现鳃细胞增生和肝细胞空泡化。阿迪亚克的鳃中增殖细胞核抗原（PCNA）阳性细胞的百分比为 45.8%±23.7%，埃布拉为 18.2%±4.6%，拉约为 11.4%±6.51%；肝组织中 PCNA 阳性细胞的百分比为阿迪亚克 3.8%±3.6%，埃布拉为 4.9%±4.7%，拉约为 2.6%±2.5%。鳃受影响比肝更严重。阿迪亚克地区是科特迪瓦泻湖复合体污染最严重的地区。
