分裂期及非分裂但正在分化的肠上皮细胞核中聚（二磷酸腺苷核糖）合成酶的活性
Poly(adenosine diphosphate ribose) synthetase activity in nuclei of dividing and of non-dividing but differentiating intestinal epithelial cells.
作者信息
Porteous J W, Furneaux H M, Pearson C K, Lake C M, Morrison A
出版信息
Biochem J. 1979 Jun 15;180(3):455-61. doi: 10.1042/bj1800455.
Abstract
Poly(ADP-ribose) synthetase activity is found in nuclei of regenerating epithelial cells in the lower half of the crypts of guinea-pig small intestine. Nuclei from non-dividing but differentiating and maturing cells in the upper crypts and on the villi contain no more than about 10% of the synthetase activity of lower-crypt cell nuclei. The product in the active nuclei is shown to be 80% poly(ADP-ribosylated) protein and 20% mono(ADP-ribosylated) protein; 60% of thetotal labelled product was attached to acid-soluble proteins (including histones), and 40% to acid-insoluble (non-histone) proteins. The average number of ADP-ribosyl units in the oligomeric chains of the poly(ADP-ribosylated) proteins was 15 but the range of sizes of (ADP-ribose) oligomers attached to nuclear proteins was smaller in villus than in crypt cell nuclei.
摘要
在豚鼠小肠隐窝下半部再生上皮细胞的细胞核中发现了聚（ADP - 核糖）合成酶活性。上隐窝和绒毛处不分裂但正在分化和成熟的细胞的细胞核，其合成酶活性不超过下隐窝细胞核的10%。活性细胞核中的产物显示为80%的聚（ADP - 核糖基化）蛋白和20%的单（ADP - 核糖基化）蛋白；总标记产物的60%附着于酸溶性蛋白（包括组蛋白），40%附着于酸不溶性（非组蛋白）蛋白。聚（ADP - 核糖基化）蛋白寡聚链中ADP - 核糖基单元的平均数量为15，但附着于核蛋白的（ADP - 核糖）寡聚物的大小范围在绒毛细胞核中比在隐窝细胞核中更小。
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本文引用的文献
1
THE ISOLATION AND CHARACTERIZATION OF SUBCELLULAR COMPONENTS OF THE EPITHELIAL CELLS OF RABBIT SMALL INTESTINE.
Biochem J. 1965 Jul;96(1):159-71. doi: 10.1042/bj0960159.
2
The isolation of purified brush borders from rat small intestine.
FEBS Lett. 1968 Jul;1(1):46-49. doi: 10.1016/0014-5793(68)80015-8.
3
Poly(adenosine diphosphate-ribose) polymerase in quail oviduct. Changes during estrogen and progesterone induction.
Nucleic Acids Res. 1974 Oct;1(10):1317-27. doi: 10.1093/nar/1.10.1317.
4
The distribution of disaccharidase activities in the villi and crypts of the small-intestinal mucosa.
Biochim Biophys Acta. 1966 Mar 7;113(3):624-6. doi: 10.1016/s0926-6593(66)80024-3.
5
Quantitative determination of enzymes in different parts of the villi and crypts of rat small intestine. Comparison of alkaline phosphatase, disaccharidases and dipepeptidases.
J Histochem Cytochem. 1967 Dec;15(12):713-21. doi: 10.1177/15.12.713.
6
The cellular localization of enterokinase.
Biochim Biophys Acta. 1970 Mar 18;198(3):621-2. doi: 10.1016/0005-2744(70)90144-0.
7
Distribution of leucyl naphthylamidase and alkaline phosphatase on the villi of the chick duodenum.
J Histochem Cytochem. 1970 Jun;18(6):416-23. doi: 10.1177/18.6.416.
8
Electron microscopy of the small intestine: a review.
Gastroenterology. 1965 Nov;49(5):574-603.
9
Variation in the duration of mitosis in the crypts of Lieberkuhn of the rat; a cytokinetic study using vincristine.
Cell Tissue Kinet. 1972 Jul;5(4):351-64. doi: 10.1111/j.1365-2184.1972.tb00374.x.
10
Studies on epithelial cells isolated from guinea pig small intestine.
J Cell Biol. 1971 Nov;51(21):452-64. doi: 10.1083/jcb.51.2.452.
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