制备 pH 敏感型聚丙烯酸酯两亲性共聚物及其在纤维素酶固定化中的应用。
Preparation of a pH-sensitive polyacrylate amphiphilic copolymer and its application in cellulase immobilization.
机构信息
State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
出版信息
Bioresour Technol. 2012 Jul;116:140-6. doi: 10.1016/j.biortech.2012.03.082. Epub 2012 Apr 10.
P(MDB), a pH-sensitive and reversible water-soluble copolymer, was synthesized with methacrylic acid (MAA), 2-(dimethylamino) ethyl methacrylate (DMAEMA), and butyl methacrylate (BMA) and used as carrier for cellulase. The copolymer is insoluble between pH 2.5 and 4.1, and soluble below pH 2.5 or above 4.1. Its recovery in aqueous solution was 97.2% by adjusting its isoelectric point (pI) to 3.1. Cellulase was covalently immobilized on P(MDB) with 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide. Under optimized conditions, the activity yield of immobilized cellulase was 63.24% and its recovery was 96.8% by adjusting the pI to 3.5. Maximum activity of the immobilized cellulase was achieved at 60 °C (pH 5.0), while free cellulase exhibited maximum activity at 55 °C (pH 5.0). The immobilized cellulase retained 83.1% of its initial activity after repeated five cycles of hydrolysis reaction. P(MDB) is a promising carrier for immobilizing enzymes with high and low optimum pH due to its dissolving characteristics.
P（MDB）是一种 pH 敏感且可还原的水溶性共聚物，由甲基丙烯酸（MAA）、2-（二甲氨基）乙基甲基丙烯酸酯（DMAEMA）和甲基丙烯酸丁酯（BMA）合成，用作纤维素酶的载体。该共聚物在 pH 2.5 至 4.1 之间不溶，在 pH 2.5 以下或 pH 4.1 以上时可溶。通过将等电点（pI）调节至 3.1，其在水溶液中的回收率为 97.2%。纤维素酶通过 1-乙基-3-（3-二甲基氨基丙基）碳二亚胺共价固定在 P（MDB）上。在优化条件下，通过将 pI 调节至 3.5，固定化纤维素酶的酶活收率为 63.24%，回收率为 96.8%。固定化纤维素酶的最大活性在 60°C（pH 5.0）下达到，而游离纤维素酶在 55°C（pH 5.0）下表现出最大活性。固定化纤维素酶在重复五次水解反应后保留了其初始活性的 83.1%。由于其溶解特性，P（MDB）是一种很有前途的固定化具有高和低最佳 pH 值的酶的载体。
Zotero 插件