Marine Biotechnology Research Center, Korea Ocean Research & Development Institute, Ansan, South Korea.
Appl Environ Microbiol. 2012 Aug;78(15):5440-3. doi: 10.1128/AEM.00844-12. Epub 2012 May 18.
We developed one-step sequence- and ligation-independent cloning (SLIC) as a simple, cost-effective, time-saving, and versatile cloning method. Highly efficient and directional cloning can be achieved by direct bacterial transformation 2.5 min after mixing any linearized vector, an insert(s) prepared by PCR, and T4 DNA polymerase in a tube at room temperature.
我们开发了一步式序列和连接独立克隆(SLIC)技术,作为一种简单、经济、省时且通用的克隆方法。通过在室温下将任何线性化载体、通过 PCR 制备的插入物(s)和 T4 DNA 聚合酶在管中直接混合,在 2.5 分钟内即可实现高效和定向克隆。