Galland S, Fayet Y, Degiuli A, Got R, Azzar G, Letoublon R, Frot-Coutaz J, Nicol M, Azais-Braesco V, Pascal G
Laboratoire de Biochimie des Membranes, Université Cl. Bernard Lyon I, Villeurbanne, France.
Food Addit Contam. 1990;7 Suppl 1:S162-7. doi: 10.1080/02652039009373873.
Liver nuclei, prepared from normal and vitamin A-deficient rats, were incubated in the presence of GDP-(14C)mannose or UDP-N-acetyl(14C)glucosamine and the labelled glycoproteins analysed by SDS PAGE. Fluorographic analysis has shown that (14C) mannose labelling is enhanced by vitamin A deficiency whereas N-acetyl(14C)glucosamine transfer remains approximately at the same level regardless of the vitamin A status; we did not notice any modification when the proteins were monitored by Coomassie blue or by silver nitrate.