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Effect of vitamin A deficiency on the in vitro transfer of mannose and N-acetylglucosamine in rat liver nuclei.

作者信息

Galland S, Fayet Y, Degiuli A, Got R, Azzar G, Letoublon R, Frot-Coutaz J, Nicol M, Azais-Braesco V, Pascal G

机构信息

Laboratoire de Biochimie des Membranes, Université Cl. Bernard Lyon I, Villeurbanne, France.

出版信息

Food Addit Contam. 1990;7 Suppl 1:S162-7. doi: 10.1080/02652039009373873.

Abstract

Liver nuclei, prepared from normal and vitamin A-deficient rats, were incubated in the presence of GDP-(14C)mannose or UDP-N-acetyl(14C)glucosamine and the labelled glycoproteins analysed by SDS PAGE. Fluorographic analysis has shown that (14C) mannose labelling is enhanced by vitamin A deficiency whereas N-acetyl(14C)glucosamine transfer remains approximately at the same level regardless of the vitamin A status; we did not notice any modification when the proteins were monitored by Coomassie blue or by silver nitrate.

摘要

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