Department of Civil and Environmental Engineering, University of Massachusetts, Amherst, MA 01003, USA.
Water Res. 2012 Sep 1;46(13):4292-300. doi: 10.1016/j.watres.2012.04.040. Epub 2012 May 5.
The goal of this study was to investigate the bacterial community in activated sludge with an anaerobic side-stream reactor (ASSR), a process permitting significant decrease in sludge production during wastewater treatment. The study operated five activated sludge systems with different sludge treatment schemes serving as various controls for the activated sludge with ASSR. Bacterial communities were analyzed by denaturing gradient gel electrophoresis (DGGE), sequencing and construction of phylogenetic relationships of the identified bacteria. The DGGE data showed that activated sludge incorporating ASSR contained higher diversity of bacteria, resulting from long solids retention time and recirculation of sludge under aerobic and anaerobic conditions. The similarity of DGGE profiles between ASSR and separate anaerobic digester (control) was high indicating that ASSR is primarily related to conventional anaerobic digesters. Nevertheless, there was also unique bacteria community appearing in ASSR. Interestingly, sludge in the main system and in ASSR showed considerably different bacterial composition indicating that ASSR allowed enriching its own bacterial community different than that from the aeration basin, although two reactors were connected via sludge recirculation. In activated sludge with ASSR, sequences represented by predominant DGGE bands were affiliated with Proteobacteria. The remaining groups were composed of Spirochaetes, Clostridiales, Chloroflexi, and Actinobacteria. Their putative role in the activated sludge with ASSR is also discussed in this study.
本研究的目的是调查在厌氧侧流反应器（ASSR）中活性污泥中的细菌群落，这一过程允许在处理废水时显著减少污泥的产生。该研究运行了五个具有不同污泥处理方案的活性污泥系统，作为各种对照来研究含有 ASSR 的活性污泥。通过变性梯度凝胶电泳（DGGE）、测序和鉴定细菌的系统发育关系来分析细菌群落。DGGE 数据表明，由于长固体停留时间和在有氧和无氧条件下污泥的再循环，含有 ASSR 的活性污泥含有更高多样性的细菌。ASSR 和单独的厌氧消化器（对照）之间的 DGGE 图谱的相似性很高，表明 ASSR 主要与传统的厌氧消化器有关。然而，ASSR 中也出现了独特的细菌群落。有趣的是，主系统和 ASSR 中的污泥显示出相当不同的细菌组成，表明 ASSR 允许富集自己的细菌群落，而不是来自曝气池的细菌群落，尽管两个反应器通过污泥再循环连接。在含有 ASSR 的活性污泥中，主要 DGGE 条带代表的序列与变形菌门有关。其余的组由螺旋体菌、梭菌目、绿弯菌门和放线菌门组成。在本研究中还讨论了它们在含有 ASSR 的活性污泥中的潜在作用。
