Allergy and Clinical Immunology Division, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21224, USA.
J Immunol Methods. 2012 Sep 28;383(1-2):54-9. doi: 10.1016/j.jim.2012.05.015. Epub 2012 May 30.
The diagnosis of human allergic disease involves an initial clinical history based association of allergic symptoms with an allergen exposure. This is followed by confirmation of sensitization (IgE antibody positivity) with skin test, serology or provocation testing. Once diagnosed, the allergic individual can be managed using one or several modalities that involve allergen avoidance, pharmacotherapy, allergen immunotherapy and anti-IgE therapy. This report examines Law of Mass Action considerations for the design of immunological methods which permit the quantification of free (non-Omalizumab bound) IgE in the serum of patients receiving anti-IgE therapy. The rationale for and design and performance of a surface plasmon resonance assay for the detection of "free" IgE (unbound with anti-IgE) is presented as an alternative to microtiter plate based ELISAs.
人类过敏性疾病的诊断涉及基于过敏症状与过敏原暴露的初始临床病史的关联。然后通过皮肤试验、血清学或激发试验来确认致敏（IgE 抗体阳性）。一旦确诊，过敏个体可以使用一种或多种方法进行管理，包括过敏原回避、药物治疗、过敏原免疫治疗和抗 IgE 治疗。本报告检查了用于设计免疫学方法的质量作用定律考虑因素，这些方法允许定量检测接受抗 IgE 治疗的患者血清中的游离（非奥马珠单抗结合）IgE。本文提出了一种基于表面等离子体共振的检测“游离”IgE（与抗 IgE 无结合）的方法，作为基于微量滴定板的 ELISA 的替代方法，介绍了该方法的原理、设计和性能。
