Ma H, Busch R H, Riera-Lizarazu O, Rines H W, Dill-Macky R
Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108, USA, US.
Theor Appl Genet. 1999 Aug;99(3-4):432-6. doi: 10.1007/s001220051254.
Anther culture and maize hybridization are two frequently used techniques for doubled haploid production in wheat (Triticum aestivum L.). Information on the field performance of lines derived from these techniques is limited. This study was conducted to compare the performance of F(4:6) lines obtained by single-seed descent with lines obtained by anther culture and maize (Zea mays L.) pollination from the same cross of spring wheat, 'Chris'/MN 7529. Thirty-three lines derived from each of those techniques were evaluated in six environments for grain yield, protein content, test weight, heading date, kernel weight and plant height. Mean performance of the single-seed descent lines exceeded performance of the anther culture lines for grain yield, kernel weight and plant height with no apparent differences for grain protein content, test weight and heading date. No differences between trait means for the single-seed descent and maize pollination lines were found except for plant height. The best 5 lines from each method for grain yield, protein content and test weight were similar in performance except that the protein content was higher for the maize pollination lines than for the single-seed descent lines. Acceptable levels of agronomic performance could be found among lines from each method. Wide acceptance of the doubled haploid technique for pure line production in breeding programs may, however, be limited by the often poor efficiency of doubled haploid line production, resulting in smaller population sizes for selection of desirable traits in comparison to the single-seed descent method.
花药培养和玉米杂交是小麦（普通小麦）双单倍体生产中常用的两种技术。关于通过这些技术获得的品系田间表现的信息有限。本研究旨在比较通过单粒传法获得的F(4:6)品系与通过花药培养和玉米（玉米）授粉从春小麦“Chris”/MN 7529的同一杂交组合中获得的品系的表现。对每种技术获得的33个品系在六种环境下进行了籽粒产量、蛋白质含量、容重、抽穗期、粒重和株高的评估。单粒传品系在籽粒产量、粒重和株高方面的平均表现超过了花药培养品系，在籽粒蛋白质含量、容重和抽穗期方面没有明显差异。除株高外，单粒传品系和玉米授粉品系的性状均值没有差异。每种方法中籽粒产量、蛋白质含量和容重最好的5个品系表现相似，只是玉米授粉品系的蛋白质含量高于单粒传品系。然而，育种计划中用于纯系生产的双单倍体技术的广泛应用可能会受到双单倍体系生产效率往往较低的限制，与单粒传法相比，选择理想性状时的群体规模较小。
