通过5'核酸酶等位基因鉴别进行单核苷酸多态性基因分型。
Genotyping of single nucleotide polymorphisms by 5' nuclease allelic discrimination.
作者信息
Malkki Mari, Petersdorf Effie W
机构信息
Fred Hutchinson Cancer Research Centre, Seattle, WA, USA.
出版信息
Methods Mol Biol. 2012;882:173-82. doi: 10.1007/978-1-61779-842-9_10.
Abstract
Real-time quantitative PCR is an efficient method for high-throughput genotyping of single nucleotide polymorphisms (SNPs). In this chapter, we describe the 5' nuclease allelic discrimination assay for genotyping biallelic SNPs.
摘要
实时定量聚合酶链反应是一种用于单核苷酸多态性（SNP）高通量基因分型的有效方法。在本章中，我们描述了用于双等位基因SNP基因分型的5'核酸酶等位基因鉴别分析方法。
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