IMGT/DomainGapAlign：用于分析免疫球蛋白（IG）、T细胞受体（TR）、主要组织相容性复合体（MH）、免疫球蛋白超家族（IgSF）和主要组织相容性复合体超家族（MhSF）结构域氨基酸多态性的IMGT®工具。

IMGT/DomainGapAlign: the IMGT® tool for the analysis of IG, TR, MH, IgSF, and MhSF domain amino acid polymorphism.

作者信息

Ehrenmann François, Lefranc Marie-Paule

机构信息

IMGT®, The International ImMunoGeneTics Information System®, Université Montpellier 2, Laboratoire d'ImmunoGénétique Moléculaire (LIGM), Institut de Génétique Humaine (IGH), UPR CNRS 1142, Montpellier, France.

出版信息

Methods Mol Biol. 2012;882:605-33. doi: 10.1007/978-1-61779-842-9_33.

DOI:10.1007/978-1-61779-842-9_33

PMID:22665257

Abstract

IMGT/DomainGapAlign is the online tool of IMGT(®), the international ImMunoGeneTics information system(®), for the analysis of amino acid sequences and two-dimensional (2D) structures of domains. IMGT/DomainGapAlign allows the analysis of the closest variable (V) and constant (C) domains of immunoglobulins (IG) or antibodies, T cell receptors (TR), and immunoglobulin superfamily (IgSF) proteins, and of the groove (G) domains of major histocompatibility (MH; in humans, HLA for human leukocyte antigen) and MH superfamily proteins. IMGT/DomainGapAlign aligns the user own sequences against the IMGT domain reference directory, displays amino acid changes, creates IMGT gaps, and delimits the domain strands and loops (and helix for G domain) according to the IMGT unique numbering. IMGT/DomainGapAlign is coupled to the IMGT/Collier-de-Perles tool that draws standardized IMGT Colliers de Perles. The analysis is based on the IMGT-ONTOLOGY concepts of identification, classification, description, and numerotation generated from the axioms of the Formal IMGT-ONTOLOGY or IMGT-Kaleidoscope. IMGT/DomainGapAlign provides an invaluable help for antibody engineering and antibody humanization as it precisely defines the standardized framework regions (FR-IMGT) and complementarity determining regions (CDR-IMGT) to be grafted. IMGT/DomainGapAlign is freely available at http://www.imgt.org.

摘要

IMGT/DomainGapAlign是国际免疫基因信息系统（IMGT(®)）的在线工具，用于分析结构域的氨基酸序列和二维（2D）结构。IMGT/DomainGapAlign可分析免疫球蛋白（IG）或抗体、T细胞受体（TR）以及免疫球蛋白超家族（IgSF）蛋白的最接近可变（V）结构域和恒定（C）结构域，以及主要组织相容性（在人类中为人类白细胞抗原HLA的MH）和MH超家族蛋白的沟槽（G）结构域。IMGT/DomainGapAlign将用户自己的序列与IMGT结构域参考目录进行比对，显示氨基酸变化，创建IMGT缺口，并根据IMGT独特编号划定结构域链和环（以及G结构域的螺旋）。IMGT/DomainGapAlign与绘制标准化IMGT Colliers de Perles的IMGT/Collier-de-Perles工具相连。该分析基于从形式化IMGT本体论或IMGT万花筒公理生成的IMGT本体论的识别、分类、描述和编号概念。IMGT/DomainGapAlign为抗体工程和抗体人源化提供了宝贵的帮助，因为它精确地定义了要移植的标准化框架区（FR-IMGT）和互补决定区（CDR-IMGT）。IMGT/DomainGapAlign可在http://www.imgt.org免费获取。