Nemo 样激酶促进依托泊苷诱导的雄性生殖细胞源性 GC-1 细胞凋亡。
Nemo-like kinase promotes etoposide-induced apoptosis of male germ cell-derived GC-1 cells in vitro.
机构信息
State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Tsinghua University, Beijing, China.
出版信息
FEBS Lett. 2012 May 21;586(10):1497-503. doi: 10.1016/j.febslet.2012.04.005. Epub 2012 Apr 18.
Spermatogenesis is an extremely intricate process that is tightly regulated and orchestrated by a series of well-coordinated gene expression programmes. Nemo-like kinase (NLK) is an evolutionarily conserved serine/threonine kinase that functions in a wide variety of developmental events. Nevertheless, the function of NLK in spermatogenesis has not been investigated. In this study, we found that the distribution of NLK in mice exhibited a dynamic change during testicular development and gradually became concentrated in the acrosomes of elongated spermatids. NLK overexpression promoted etoposide-induced apoptosis of male germ cell-derived GC-1 cells, while knockdown of NLK by RNA interference (RNAi) attenuated etoposide-induced apoptosis. Our findings suggest that NLK plays an important role in etoposide-induced germ cell apoptosis and may be associated with spermatogenesis.
精子发生是一个极其复杂的过程，受到一系列协调良好的基因表达程序的严格调控。Nemo 样激酶（NLK）是一种进化上保守的丝氨酸/苏氨酸激酶，在多种发育事件中发挥作用。然而，NLK 在精子发生中的功能尚未被研究过。在这项研究中，我们发现 NLK 在小鼠睾丸发育过程中的分布呈现动态变化，逐渐集中在长形精子的顶体中。NLK 的过表达促进依托泊苷诱导的雄性生殖细胞源性 GC-1 细胞凋亡，而 RNA 干扰（RNAi）敲低 NLK 则减弱了依托泊苷诱导的凋亡。我们的研究结果表明，NLK 在依托泊苷诱导的生殖细胞凋亡中发挥重要作用，可能与精子发生有关。
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