Nielsen Peter E
Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Artif DNA PNA XNA. 2012 Jan-Mar;3(1):1-2. doi: 10.4161/adna.19672.
The recent claim by Wolfe-Simon et al. that the Halomonas bacterial strain GFAJ-1 when grown in arsenate-containing medium with limiting phosphate is able to substitute phosphate with arsenate in biomolecules including nucleic acids and in particular DNA(1) arose much skepticism, primarily due to the very limited chemical stability of arsenate esters (see ref. 2 and references therein). A major part of the criticisms was concerned with the insufficient (bio)chemical evidence in the Wolfe-Simon study for the actual chemical incorporation of arsenate in DNA (and/or RNA). Redfield et al. now present evidence that the identification of arsenate DNA was artifactual.
最近，沃尔夫-西蒙等人宣称，嗜盐单胞菌菌株GFAJ-1在含砷酸盐且磷酸盐受限的培养基中生长时，能够在包括核酸尤其是DNA在内的生物分子中用砷酸盐替代磷酸盐(1)，这引发了诸多质疑，主要原因是砷酸酯的化学稳定性非常有限(见参考文献2及其中的参考文献)。大部分批评集中在沃尔夫-西蒙研究中缺乏足够的(生物)化学证据来证明砷酸盐实际化学掺入了DNA(和/或RNA)。雷德菲尔德等人现在提供证据表明，对砷酸盐DNA的鉴定是人为造成的。
