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鉴定和分析库蚊(双翅目:蚊科)中的单核苷酸多态性(SNPs)。

Identification and characterization of single nucleotide polymorphisms (SNPs) in Culex theileri (Diptera: Culicidae).

机构信息

Hacettepe University, Faculty of Science, Department of Biology, 06800 Beytepe-Ankara, Turkey.

出版信息

J Med Entomol. 2012 May;49(3):581-8. doi: 10.1603/me11139.

Abstract

Culex theileri Theobald (Diptera: Culicidae) is one of the most common mosquito species in northeastern Turkey and serves as a vector for various zoonotic diseases including West Nile virus. Although there have been some studies on the ecology of Cx. theileri, very little genetic data has been made available. We successfully sequenced 11 gene fragments from Cx. theileri specimens collected from the northeastern part of Turkey. On average, we found a Single nucleotide polymorphism every 45 bp. Transitions outnumbered transversions, at a ratio of 2:1. This is the first report of genetic polymorphisms in Cx. theileri and Single nucleotide polymorphism discovered from this study can be used to investigate population structure and gene-environmental interactions.

摘要

Theileri 库蚊(Theobald)(双翅目:蚊科)是土耳其东北部最常见的蚊子之一,是包括西尼罗河病毒在内的各种人畜共患病的媒介。尽管已经有一些关于 Theileri 库蚊生态学的研究,但很少有遗传数据可用。我们成功地从土耳其东北部采集的 Theileri 库蚊标本中测序了 11 个基因片段。平均而言,我们每 45bp 发现一个单核苷酸多态性。转换多于颠换,比例为 2:1。这是首次报道 Theileri 库蚊的遗传多态性,本研究发现的单核苷酸多态性可用于研究种群结构和基因-环境相互作用。

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