Onetti Muda A, Crescenzi A, Faraggiana T, Marinozzi V
Dipartimento di Biopatologia Umana, Università La Sapienza, Roma, Italy.
Basic Appl Histochem. 1990;34(3):189-97.
A non-specific staining of mast cells by a number of proteins and immunoglobulins has been observed using different immunocytochemical techniques and different antisera at light and electron microscopy level. This finding has been related to an ionic binding of cationic charges, such as aminic groups with an high pI, to the strongly anionic residues of mast cell granules. This hypothesis is supported by the lack of non-specific binding achieved using negatively charged chromogens (i.e. FITC--or Rhodamine-conjugated antibodies) or using cationic dyes before the immunohistochemical reaction. It is therefore stressed that adequate and accurate negative controls must always be carried out in order to get correct interpretations of the staining results.
使用不同的免疫细胞化学技术和不同抗血清，在光学和电子显微镜水平下，已观察到多种蛋白质和免疫球蛋白对肥大细胞的非特异性染色。这一发现与阳离子电荷（如具有高pI的氨基基团）与肥大细胞颗粒的强阴离子残基的离子结合有关。使用带负电荷的显色剂（即异硫氰酸荧光素或罗丹明偶联抗体）或在免疫组织化学反应前使用阳离子染料均未实现非特异性结合，这支持了该假设。因此强调，为了正确解释染色结果，必须始终进行充分且准确的阴性对照。
