基于微阵列的成年斑马鱼光损伤视网膜中穆勒胶质细胞衍生的视网膜干细胞的基因谱分析。
Microarray-based gene profiling analysis of Müller glia-derived retinal stem cells in light-damaged retinas from adult zebrafish.
作者信息
Qin Zhao, Raymond Pamela A
机构信息
Department of Molecular, Cellular, and Developmental Biology, University of Michigan College of Literature, Science, and the Arts, Ann Arbor, MI, USA.
出版信息
Methods Mol Biol. 2012;884:255-61. doi: 10.1007/978-1-61779-848-1_18.
Abstract
Microarray-based gene profiling has become an important technique to measure changes in gene expression on a genome-wide scale. Recently, cell-specific microarrays have been reported to study changes in gene expression of a particular cell type in several model organisms. Here, we describe a protocol to prepare RNA samples for microarray analysis of isolated Müller glia-derived retinal stem cells from light-damaged adult zebrafish expressing a fluorescent marker in Müller cells using enzymatic retinal dissociation followed by fluorescence-activated cell sorting (FACS).
摘要
基于微阵列的基因谱分析已成为在全基因组范围内测量基因表达变化的一项重要技术。最近，据报道，细胞特异性微阵列可用于研究几种模式生物中特定细胞类型的基因表达变化。在此，我们描述了一种制备RNA样本的方案，用于对来自光损伤成年斑马鱼的分离的穆勒胶质细胞衍生的视网膜干细胞进行微阵列分析，这些成年斑马鱼的穆勒细胞中表达荧光标记，采用酶促视网膜解离，随后进行荧光激活细胞分选（FACS）。
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