球形红杆菌产氢活性与质子动力和 F(0)F(1)-ATP 酶的关系：二苯并碘鎓（一种氢化酶抑制剂）及其溶剂二甲亚砜的影响。
Relationship of proton motive force and the F(0)F (1)-ATPase with bio-hydrogen production activity of Rhodobacter sphaeroides: effects of diphenylene iodonium, hydrogenase inhibitor, and its solvent dimethylsulphoxide.
机构信息
Department of Biophysics, Yerevan State University, 0025, Yerevan, Armenia.
出版信息
J Bioenerg Biomembr. 2012 Aug;44(4):495-502. doi: 10.1007/s10863-012-9450-3. Epub 2012 Jun 12.
Rhodobacter sphaeroides MDC 6521 was able to produce bio-hydrogen (H(2)) in anaerobic conditions under illumination. In this study the effects of the hydrogenase inhibitor-diphenylene iodonium (Ph(2)I) and its solvent dimethylsulphoxide (DMSO) on growth characteristics and H(2) production by R. sphaeroides were investigated. The results point out the concentration dependent DMSO effect: in the presence of 10 mM DMSO H(2) yield was 6 fold lower than that of the control. The bacterium was unable to produce H(2) in the presence of Ph(2)I. In order to examine the mediatory role of proton motive force (∆p) or the F(0)F(1)-ATPase in H(2) production by R. sphaeroides, the effects of Ph(2)I and DMSO on ∆p and its components (membrane potential (∆ψ) and transmembrane pH gradient), and ATPase activity were determined. In these conditions ∆ψ was of -98 mV and the reversed ∆pH was +30 mV, resulting in ∆p of -68 mV. Ph(2)I decreased ∆ψ in concentrations of 20 μM and higher; lower concentrations of Ph(2)I as DMSO had no valuable effect on ∆ψ. The R. sphaeroides membrane vesicles demonstrated significant ATPase activity sensitive to N,N'-dicyclohexylcarbodiimide. The 10-20 μM Ph(2)I did not affect the ATPase activity, whereas 40 μM Ph(2)I caused a marked inhibition (2 fold) in ATPase activity. The obtained results provide novel evidence on the involvement of hydrogenase and the F(0)F(1)-ATPase in H(2) production by R. sphaeroides. Moreover, these data indicate the role of hydrogenase and the F(0)F(1)-ATPase in ∆p generation. In addition, DMSO might increase an interaction of nitrogenase with CO(2), decreasing nitrogenase activity and affecting H(2) production.
球形红杆菌 MDC 6521 能够在光照厌氧条件下生产生物氢（H 2 ）。在这项研究中，研究了氢酶抑制剂二苯基碘鎓（Ph 2 I）及其溶剂二甲亚砜（DMSO）对球形红杆菌生长特性和 H 2 生产的影响。结果表明 DMSO 具有浓度依赖性：在 10mM DMSO 的存在下，H 2 的产率比对照低约 6 倍。在 Ph 2 I 的存在下，细菌无法产生 H 2 。为了检验质子动力势（∆p）或 F 0 F 1 -ATPase 在球形红杆菌 H 2 生产中的中介作用，测定了 Ph 2 I 和 DMSO 对∆p 及其组成部分（膜电位（∆ψ）和跨膜 pH 梯度）和 ATPase 活性的影响。在这些条件下，∆ψ 为-98 mV，反向 ∆pH 为+30 mV，导致∆p 为-68 mV。Ph 2 I 在 20 μM 及更高浓度下降低∆ψ；较低浓度的 Ph 2 I 与 DMSO 对∆ψ 没有有价值的影响。球形红杆菌膜囊泡表现出对 N，N'-二环己基碳二亚胺敏感的显著 ATPase 活性。10-20 μM Ph 2 I 不影响 ATPase 活性，而 40 μM Ph 2 I 导致 ATPase 活性明显抑制（~2 倍）。所得结果提供了氢酶和 F 0 F 1 -ATPase 参与球形红杆菌 H 2 生产的新证据。此外，这些数据表明氢酶和 F 0 F 1 -ATPase 在∆p 产生中的作用。此外，DMSO 可能会增加固氮酶与 CO 2 的相互作用，降低固氮酶活性并影响 H 2 的产生。
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