Bondarenko V M, Rybal'chenko O V, Orlova O G, Fialkina S V
Zh Mikrobiol Epidemiol Immunobiol. 2012 Mar-Apr(2):3-7.
Determination of morpho-physiological changes in cells of a lactobacillus industry strain suppressed by antagonistically active clinical cultures of Staphylococcus aureus.
5 clinical strains of Staphylococcus aureus detected among 42 strains isolated previously in atopic dermatitis were used. Bacteria of the industry strain Lactobacillus plantarum 8PA-3 that synthesize bacteriocin-like inhibitory substances (BLIS) were used as target-cells. Suppression of growth of lactobacilli cells by staphylococci was performed on modified agar nutrient medium MRS by delayed antagonism method. Ultrastructure changes in lactobacilli cells exposed to staphylococci BLIS were studied by various methods of transmission electron microscopy.
Destructive changes (exfoliation of small layered fragments of peptidoglycan layers) were detected in the cell walls of lactobacilli suppressed by staphylococci BLIS, and rearrangement of ultrathin structure of their cytoplasm with the destruction of protein-ribosomal complex was noticed. CONCLUSION; Clinical S. aureus strains that produce BLIS, suppress antagonistic activity of lactobacilli were revealed, and morpho-physiological changes in lactobacilli target-cells damaged by BLIS were detected. Local damage of cell components manifested by the destruction of cytoplasm and formation of "cell shadows".
确定被具有拮抗活性的金黄色葡萄球菌临床培养物抑制的乳酸杆菌工业菌株细胞的形态生理变化。
使用先前从特应性皮炎中分离出的42株菌株中检测到的5株金黄色葡萄球菌临床菌株。合成类细菌素抑制物质（BLIS）的植物乳杆菌工业菌株8PA - 3的细菌用作靶细胞。通过延迟拮抗法在改良的琼脂营养培养基MRS上进行葡萄球菌对乳酸杆菌细胞生长的抑制。通过各种透射电子显微镜方法研究暴露于葡萄球菌BLIS的乳酸杆菌细胞的超微结构变化。
在被葡萄球菌BLIS抑制的乳酸杆菌细胞壁中检测到破坏性变化（肽聚糖层的小分层片段剥落），并且注意到其细胞质的超薄结构重新排列以及蛋白质 - 核糖体复合物的破坏。结论：发现产生BLIS的金黄色葡萄球菌临床菌株抑制乳酸杆菌的拮抗活性，并检测到被BLIS损伤的乳酸杆菌靶细胞的形态生理变化。细胞成分的局部损伤表现为细胞质的破坏和“细胞阴影”的形成。
