Erciyes University, Medical Faculty, Department of Medical Biology, 38039 Kayseri, Turkey.
Mutat Res. 2012 Oct 9;748(1-2):1-7. doi: 10.1016/j.mrgentox.2012.05.013. Epub 2012 Jun 12.
The cytokinesis-block micronucleus cytome (CBMN-Cyt) assay was originally developed as an ideal system for measuring DNA damage, cytostasis and cytotoxicity. The objective of the present study is to simultaneously evaluate the background levels of micronuclei (MN), nucleoplasmic bridges (NPBs), nuclear buds (NBUDs), cell death (necrosis or apoptosis) and nuclear division index (NDI) in the peripheral blood lymphocytes of non-occupationally exposed, healthy subjects living in the city of Kayseri in Turkey. We used the CBMN-Cyt assay, taking into account factors - age, gender, and smoking habits - that might affect MN frequency and also other CBMN-Cyt assay parameters. Ninety-six healthy subjects (48 female and 48 male) were selected with ages varying between 21 and 60 years. The parameters, except for the number of binucleated (BN) cells with NPBs, showed no statistically significant difference between smokers and non-smokers. There were significant differences between female and male groups in MN frequency (higher in females) and in the number of NPBs (lower in females), while the other parameters were not significantly different between genders. The correlations between years of age and MN frequency, number of NPBs and the frequency of necrotic cells were statistically significant, while the correlations between the years of age and the other parameters were not. The results of the correlation analysis between years of smoking and MN frequency were positive, although no statistically significant correlation was found between the years of smoking and the other parameters. Among the smokers, no correlation was found either between the pack-years of smoking and the parameters assessed in this group. The results of the present study provide evidence of increasing MN frequency, number of NPBs and frequency of necrotic cells with increasing age in the peripheral blood lymphocytes of healthy individuals and influencing MN frequency and number of NPBs by gender.
有丝分裂阻断微核细胞胞质分裂（CBMN-Cyt）试验最初被开发为测量 DNA 损伤、细胞停滞和细胞毒性的理想系统。本研究的目的是同时评估土耳其开塞利市非职业暴露健康个体外周血淋巴细胞中的微核（MN）、核质桥（NPB）、核芽（NBUD）、细胞死亡（坏死或凋亡）和核分裂指数（NDI）的背景水平。我们使用 CBMN-Cyt 试验，考虑到可能影响 MN 频率和其他 CBMN-Cyt 试验参数的因素，如年龄、性别和吸烟习惯。选择了 96 名健康个体（48 名女性和 48 名男性），年龄在 21 至 60 岁之间。除了具有 NPB 的双核细胞（BN）数量外，吸烟和不吸烟组之间的参数没有统计学差异。女性组和男性组之间 MN 频率（女性较高）和 NPB 数量（女性较低）存在显著差异，而其他参数在性别之间无显著差异。年龄与 MN 频率、NPB 数量和坏死细胞频率之间存在统计学显著相关性，而年龄与其他参数之间没有相关性。吸烟年限与 MN 频率之间的相关性分析结果为正相关，尽管吸烟年限与其他参数之间未发现统计学显著相关性。在吸烟者中，吸烟年限与该组评估的参数之间也未发现相关性。本研究结果提供了证据，表明健康个体外周血淋巴细胞中的 MN 频率、NPB 数量和坏死细胞频率随年龄增长而增加，并受性别影响。
