Li Hui-Min, Guo Li, Zeng De-Long, Guan Qing-Xin, Wu Zheng-Jun, Qin Xin-Min
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, College of Life Sciences, Guangxi Normal University, Guilin 541004, PR China.
Mitochondrial DNA. 2012 Aug;23(4):315-7. doi: 10.3109/19401736.2012.683186. Epub 2012 Jun 18.
The 16,585 base pairs mitochondrial genome of Shinisaurus crocodilurus was determined by using PCR amplification and DNA sequencing. To determine the phylogenetic position of S. crocodilurus with related species within Squamata, the phylogenetic tree was reconstructed with the concatenated nucleotide sequences of the 12 heavy-strand-encoded protein genes. Phylogenetic analyses based on maximum parsimony and Bayesian inference methods consistently support that the S. crocodilurus was closely related to the Helodermatidae within a monophyletic Anguimorpha group. And the result here contradicted the monophyly of Varanoidea (Varanidae + Helodermatidae). In addition, the Gekkonidae was found to possess a basal phylogenetic position within squamata and the traditional hypothesis of monophyletic lineages of Iguania and Scleroglossa was not supported in this study.
通过PCR扩增和DNA测序确定了鳄蜥16,585个碱基对的线粒体基因组。为了确定鳄蜥在有鳞目内与相关物种的系统发育位置,利用12个重链编码蛋白基因的串联核苷酸序列重建了系统发育树。基于最大简约法和贝叶斯推断方法的系统发育分析一致支持鳄蜥在单系的蛇蜥亚目类群中与毒蜥科密切相关。并且这里的结果与巨蜥超科(巨蜥科+毒蜥科)的单系性相矛盾。此外,发现壁虎科在有鳞目中具有基部系统发育位置,并且本研究不支持鬣蜥亚目和硬舌亚目的单系谱系的传统假说。