Department of Pathology, XiangYa School of Medicine, Central South University, Changsha, Hunan Province, China.
APMIS. 2012 Jul;120(7):582-90. doi: 10.1111/j.1600-0463.2012.02874.x. Epub 2012 Mar 2.
We have previously shown that overexpression of RASSF1A inhibits the growth of human gastric cancer SGC7901 cells, but the underlying mechanism remains unknown. In this study, the differential protein expression by RASSF1A gene in human gastric cancer cell line SGC7901 was determined by 2-D gel electrophoresis combined with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and bioinformatics. Differential expression analysis of the protein profiles by RASSF1A gene identified a total of 35 protein spots, of which 10 were up-regulated and 25 were down-regulated. Eight proteins were identified by MALDI-TOF MS: Galectin-1, TRP-14, ACBP, PSMB5, PSMB4, TIM, vimentin, CD79α. RASSF1A up-regulated the mRNA expression of Galectin-1, TRP-14, ABCP in SGC7901. RASSF1A also led to an increased expression of Galectin-1 protein in SGC7901 confirmed by western blotting and immunocytochemistry analysis. RASSF1A inhibited the activity of NF-κB in SGC7901 cells. These data indicated that Galectin-1 may be playing a role in RASSF1A signaling in SGC7901.
我们之前已经证明 RASSF1A 的过表达可以抑制人胃癌 SGC7901 细胞的生长，但潜在的机制尚不清楚。在这项研究中，通过二维凝胶电泳结合基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）和生物信息学，确定了 RASSF1A 基因在人胃癌细胞系 SGC7901 中的差异蛋白表达。RASSF1A 基因对蛋白图谱的差异表达分析共鉴定出 35 个蛋白点，其中 10 个上调，25 个下调。通过 MALDI-TOF MS 鉴定出 8 种蛋白质：半乳糖凝集素-1、TRP-14、ACBP、PSMB5、PSMB4、TIM、波形蛋白、CD79α。RASSF1A 上调 SGC7901 中 Galectin-1、TRP-14、ACBP 的 mRNA 表达。RASSF1A 还导致 SGC7901 中 Galectin-1 蛋白表达增加，Western blot 和免疫细胞化学分析证实了这一点。RASSF1A 抑制了 SGC7901 细胞中 NF-κB 的活性。这些数据表明，Galectin-1 可能在 SGC7901 中的 RASSF1A 信号通路中发挥作用。
Zotero 插件