Gramajo-Bühler M C, Pucci-Alcaide F, Sánchez-Toranzo G
Departamento de Biología del Desarrollo, Chacabuco 461, 4000 San Miguel de Tucumán, Tucumán, Argentina.
Zygote. 2013 Nov;21(4):351-7. doi: 10.1017/S0967199412000068. Epub 2012 May 2.
Chinchilla lanigera is an endangered species therefore the development of cryopreservation protocols for its gametes is a useful tool in the application of assisted reproduction techniques. A study of the functionality of the spermatozoa punctured from the cauda epididymis was performed on fresh or frozen-thawed samples with three cryoprotective media (test-yolk buffer, sucrose and glycerol). The effect that these media had on sperm physiology during the freezing, storage and later thawing process was analysed. A decrease in the percentages of viability, motility, membrane integrity and capacity to undergo the induced acrosome reaction was found with all the media assayed, an increase in the percentages of DNA fragmentation was also observed. The comparative analysis of the effect of the different cryoprotectants assayed showed that the best medium to use to cryopreserve epididymal sperm in this species is test-yolk buffer. This medium had the least effect on the abovementioned physiological parameters, especially at the level of genetic material.
青紫蓝兔是一种濒危物种，因此开发其配子的冷冻保存方案是辅助生殖技术应用中的一项有用工具。对从附睾尾部采集的新鲜或冻融精子样本，使用三种冷冻保护剂（试验卵黄缓冲液、蔗糖和甘油）进行了精子功能研究。分析了这些保护剂在冷冻、储存及随后解冻过程中对精子生理的影响。在所检测的所有保护剂中，均发现活力、运动性、膜完整性及诱导顶体反应能力的百分比下降，同时也观察到DNA片段化百分比增加。对所检测的不同冷冻保护剂效果的比较分析表明，用于该物种附睾精子冷冻保存的最佳保护剂是试验卵黄缓冲液。该保护剂对上述生理参数影响最小，尤其是在遗传物质层面。
