Chen Kuen-Bao, Kuo Eva Yuhua, Poon Kin-Shing, Cheng Ka-Shun, Chang Chia-Sheng, Liu Yu-Cheng, Lai Ted Weita
China Medical University Hospital, China Medical University, Taichung, Taiwan.
Neuroreport. 2012 Aug 22;23(12):699-701. doi: 10.1097/WNR.0b013e3283556dcc.
The development of the blood-brain barrier (BBB) against permeability to inert tracers, such as Evans blue dye (EBD), occurs quite early on at embryonic stages (before E13-E15), and the BBB remains resistant to EBD between E15 and early adulthood (P20-P30). Here, we aimed to examine the changes in EBD permeability at a later stage in development, specifically comparing young rats (P20) with adult rats (P86). We found markedly higher EBD extravasation into the forebrains of adult rats compared with those of the young rats (P=0.0132; Student's t-test). In contrast, there was no difference in EBD extravasation to the liver, suggesting no change in vascular permeability in peripheral tissues. Furthermore, EBD extravasation into the cerebellum was less prominent than that into the forebrain, suggesting that the disruption of the BBB was brain-region specific. In conclusion, we found a specific increase in EBD extravasation in the mature forebrain, and the protocol that we used may be a good template for studying developmental disruption of the BBB.
血脑屏障（BBB）对伊文思蓝染料（EBD）等惰性示踪剂的通透性在胚胎期（E13 - E15之前）很早就开始发育，并且在E15到成年早期（P20 - P30）期间血脑屏障对EBD仍具有抗性。在此，我们旨在研究发育后期EBD通透性的变化，具体是比较幼鼠（P20）和成年大鼠（P86）。我们发现，与幼鼠相比，成年大鼠前脑的EBD外渗明显更高（P = 0.0132；学生t检验）。相比之下，EBD向肝脏的外渗没有差异，表明外周组织的血管通透性没有变化。此外，EBD向小脑的外渗不如向前脑那么明显，这表明血脑屏障的破坏具有脑区特异性。总之，我们发现成熟前脑的EBD外渗有特定增加，并且我们使用的方案可能是研究血脑屏障发育破坏的良好模板。
