Department of Gastroenterology, Infectious Diseases, and Rheumatology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Ann N Y Acad Sci. 2012 Jul;1258:100-7. doi: 10.1111/j.1749-6632.2012.06609.x.
The aim of this study was to analyze the influence of quercetin on intestinal barrier function using the human colonic epithelial cell line HT-29/B6 and rat small and large intestine in vitro. Rat native ileum and late distal colon were incubated in Ussing chambers, and the total resistance (R(T) ) was measured, and expression of tight junction proteins was characterized in immunoblots. By simulating inflammatory conditions with TNF-α, we examined the barrier-preventive effects of quercetin. Incubation with TNF-α led to a decrease of R(T) in HT-29/B6 cell monolayers, which could be partially inhibited by quercetin. In accordance with cell culture experiments, quercetin increased mucosal resistance of rat ileum and late distal colon. Thus, barrier disturbance in late distal colon specimens induced by TNF-α and IFN-γ could be partially prevented by coincubation with quercetin. These findings demonstrate that quercetin enhances barrier function in rat small and large intestine and possesses protective effects on cytokine-induced barrier damage.
本研究旨在使用人结肠上皮细胞系 HT-29/B6 和大鼠小肠和大肠在体外分析槲皮素对肠道屏障功能的影响。将大鼠固有回肠和远段结肠在 Ussing 室中孵育，并测量总电阻 (R(T) )，并通过免疫印迹法对紧密连接蛋白的表达进行特征分析。通过用 TNF-α模拟炎症条件，我们检查了槲皮素的屏障预防作用。TNF-α孵育导致 HT-29/B6 细胞单层的 R(T) 降低，而槲皮素可部分抑制该作用。与细胞培养实验一致，槲皮素增加了大鼠回肠和远段结肠的粘膜阻力。因此，用 TNF-α和 IFN-γ诱导的远段结肠标本的屏障障碍可以通过与槲皮素共孵育部分预防。这些发现表明，槲皮素增强了大鼠小肠和大肠的屏障功能，并对细胞因子诱导的屏障损伤具有保护作用。
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