School of Municipal & Environmental Engineering, Harbin Institute of Technology, 202 Haihe Road, Nangang District, Harbin 150090, China.
Water Res. 2012 Sep 15;46(14):4387-96. doi: 10.1016/j.watres.2012.05.049. Epub 2012 Jun 6.
Correlation between fractional, biodegradable and spectral characteristics of sludge extracellular polymeric substances (EPS) by different protocols has not been well established. This work extracted sludge EPS using alkaline extractants (NH₄OH and formaldehyde + NaOH) and physical protocols (ultrasonication, heating at 80 °C or cation exchange resin (CER)) and then fractionated the extracts using XAD-8/XAD-4 resins. The alkaline extractants yielded more sludge EPS than the physical protocols. However, the physical protocols extracted principally the hydrophilic components which were readily biodegradable by microorganisms. The alkaline extractants dissolved additional humic-like substances from sludge solids which were refractory in nature. Different extraction protocols preferably extracted EPS with distinct fractional, biodegradable and spectral characteristics which could be applied in specific usages.
不同方案提取的污泥胞外聚合物（EPS）的分数、可生物降解和光谱特性之间的相关性尚未得到很好的确立。本工作使用碱性提取剂（NH₄OH 和甲醛+NaOH）和物理方案（超声、80°C 加热或阳离子交换树脂（CER））提取污泥 EPS，然后使用 XAD-8/XAD-4 树脂对提取物进行分级。碱性提取剂比物理方案产生了更多的污泥 EPS。然而，物理方案主要提取了微生物易生物降解的亲水性成分。碱性提取剂从污泥固体中溶解了额外的腐殖样物质，这些物质性质上是难降解的。不同的提取方案优先提取具有不同分数、可生物降解和光谱特性的 EPS，这些特性可应用于特定用途。
