Department of Veterinary Medical Science, University of Bologna, Ozzano Emilia, (Bologna), Italy.
J Comp Neurol. 2013 Feb 1;521(2):342-66. doi: 10.1002/cne.23177.
Porcine lumbosacral dorsal root ganglion (DRG) neurons were neurochemically characterized by using six neuronal markers: calcitonin gene-related peptide (CGRP), substance P (SP), neuronal nitric oxide synthase (nNOS), neurofilament 200kDa (NF200), transient receptor potential vanilloid 1 (TRPV1), and isolectin B4 (IB4) from Griffonia simplicifolia. In addition, the phenotype and cross-sectional area of DRG neurons innervating the urinary bladder trigone (UBT) were evaluated by coupling retrograde tracer technique and immunohistochemistry. Lumbar and sacral DRG neuronal subpopulations were immunoreactive (IR) for CGRP (30 ± 3% and 29 ± 3%, respectively), SP (26 ± 8% and 27 ± 12%, respectively), nNOS (21 ± 4% and 26 ± 7%, respectively), NF200 (75 ± 14% and 81 ± 7%, respectively), and TRPV1 (48 ± 13% and 43 ± 6%, respectively), and labeled for IB4 (56 ± 6% and 43 ± 10%, respectively). UBT sensory neurons, which were distributed from L2 to Ca1 DRG, had a segmental localization, showing their highest density in L4-L5 and S2-S4 DRG. Lumbar and sacral UBT sensory neurons expressed similar percentages of NF200 immunoreactivity (64 ± 33% and 58 ± 12%, respectively) but showed a significantly different immunoreactivity for CGRP, SP, nNOS, and TRPV1 (56 ± 9%, 39 ± 15%, 17 ± 13%, 62 ± 10% vs. 16 ± 6%, 16 ± 11%, 6 ± 1%, 45 ± 24%, respectively). Lumbar and sacral UBT sensory neurons also showed different IB4 labeling (67 ± 19% and 48 ± 16, respectively). Taken together, these data indicate that the lumbar and sacral pathways probably play different roles in sensory transmission from the UBT. The findings related to cell size also reinforced this hypothesis, because lumbar UBT sensory neurons were significantly larger than sacral ones (1,112 ± 624 μm(2) vs. 716 ± 421 μm(2) ).
采用 6 种神经元标志物（降钙素基因相关肽（CGRP）、P 物质（SP）、神经元型一氧化氮合酶（nNOS）、神经丝 200kDa（NF200）、香草素受体 1（TRPV1）和荆豆凝集素 B4（IB4））对猪的腰骶背根神经节（DRG）神经元进行神经化学特征分析。此外，通过逆行示踪技术和免疫组织化学结合的方法，评估了支配膀胱三角（UBT）的 DRG 神经元的表型和横截面积。腰椎和骶椎 DRG 神经元亚群对 CGRP（分别为 30±3%和 29±3%）、SP（分别为 26±8%和 27±12%）、nNOS（分别为 21±4%和 26±7%）、NF200（分别为 75±14%和 81±7%）和 TRPV1（分别为 48±13%和 43±6%）具有免疫反应性（IR），并且对 IB4（分别为 56±6%和 43±10%）具有标记。分布于 L2 至 Ca1 DRG 的 UBT 感觉神经元具有节段性定位，在 L4-L5 和 S2-S4 DRG 中具有最高密度。腰椎和骶椎 UBT 感觉神经元表达相似百分比的 NF200 免疫反应性（分别为 64±33%和 58±12%），但 CGRP、SP、nNOS 和 TRPV1 的免疫反应性明显不同（分别为 56±9%、39±15%、17±13%、62±10%与 16±6%、16±11%、6±1%、45±24%）。腰椎和骶椎 UBT 感觉神经元的 IB4 标记也不同（分别为 67±19%和 48±16%）。综上所述，这些数据表明，腰骶通路可能在 UBT 的感觉传递中发挥不同的作用。与细胞大小相关的发现也强化了这一假设，因为腰椎 UBT 感觉神经元明显大于骶椎 UBT 感觉神经元（分别为 1,112±624μm2和 716±421μm2）。
