通过化学发光成像法无标记高灵敏检测癌细胞中端粒酶活性。
Label-free highly sensitive detection of telomerase activity in cancer cell by chemiluminescence imaging.
机构信息
School of Biological Science and Technology, Central South University, Changsha 410013, China.
出版信息
Mol Cell Probes. 2012 Oct;26(5):212-4. doi: 10.1016/j.mcp.2012.06.002. Epub 2012 Jun 26.
PMID:22750567
Abstract
We have developed a new methodology for label-free highly sensitive telomerase activity assay using chemiluminescence imaging. This method can detect the telomerase activity from as little as 10 cultured cancer cells without PCR. Furthermore, telomerase inhibition is shown, demonstrating the potential for screening of telomerase inhibitors as anticancer drug agents.
摘要
我们开发了一种新的无标记高灵敏度端粒酶活性检测方法，使用化学发光成像。该方法无需 PCR 即可检测少至 10 个培养的癌细胞中的端粒酶活性。此外，还显示了端粒酶抑制作用，表明该方法有可能筛选端粒酶抑制剂作为抗癌药物。
Zotero 插件