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皮质类固醇暴露后人晶状体上皮细胞中细胞粘附分子的表达

Cell adhesion molecule expression in human lens epithelial cells after corticosteroid exposure.

作者信息

Celojevic D, Carlsson T, Johansson Br, Nannmark U, Petersen A

机构信息

Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation/Ophthalmology.

出版信息

Open Ophthalmol J. 2012;6:42-8. doi: 10.2174/1874364101206010042. Epub 2012 Jun 21.

DOI:10.2174/1874364101206010042
PMID:22754600
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3386511/
Abstract

AIM

The aim of the study was to investigate changes in cell adhesion molecule expression in human lens epithelial cells (HLEC) subjected to glucocorticoids.

METHODS

Human lens epithelial cells were exposed to different concentrations of dexamethasone for 24 hours. Cell adhesion molecule expression was studied by western blot and immunohistochemistry of vimentin, N-cadherin, E-cadherin, α-catenin, β-catenin and γ-catenin. Expression of the glucocorticoid receptor (GR) was also studied. Cell morphology was examined by transmission electron microscopy (TEM).

RESULT

Expression of N-cadherin, α-catenin, β-catenin and GR was significantly decreased in dexamethasone exposed cells as compared to unexposed cells. No significant change in γ-catenin was present. Visualization of adhesion molecules, N-cadherin and α-catenin, by immunohistochemistry showed decreased antigen reactivity in dexamethasone exposed as compared to the unexposed cells. However, no change was seen for β-catenin and γ-catenin. E-cadherin was not detectable using western blot or immunohistochemistry. TEM showed multilayering of cells, vacuole formation and appearance of electron-dense multivesicular bodies in HLEC exposed to 0, 0.1, 1, 10 and 100 αM dexamethasone.

CONCLUSION

Glucocorticoids affect several adhesion molecules in lens epithelial cells, something that may contribute to the pathogenesis of posterior subcapsular opacification.

摘要

目的

本研究旨在调查糖皮质激素作用下人晶状体上皮细胞（HLEC）中细胞黏附分子表达的变化。

方法

将人晶状体上皮细胞暴露于不同浓度的地塞米松中24小时。通过蛋白质免疫印迹法以及波形蛋白、N-钙黏蛋白、E-钙黏蛋白、α-连环蛋白、β-连环蛋白和γ-连环蛋白的免疫组织化学研究细胞黏附分子的表达。还研究了糖皮质激素受体（GR）的表达。通过透射电子显微镜（TEM）检查细胞形态。

结果

与未暴露细胞相比，暴露于地塞米松的细胞中N-钙黏蛋白、α-连环蛋白、β-连环蛋白和GR的表达显著降低。γ-连环蛋白无显著变化。通过免疫组织化学观察黏附分子N-钙黏蛋白和α-连环蛋白，与未暴露细胞相比，暴露于地塞米松的细胞中抗原反应性降低。然而，β-连环蛋白和γ-连环蛋白未见变化。使用蛋白质免疫印迹法或免疫组织化学未检测到E-钙黏蛋白。透射电子显微镜显示，暴露于0、0.1、1、10和100αM地塞米松的人晶状体上皮细胞出现细胞多层化、液泡形成和电子致密多囊泡体。

结论

糖皮质激素影响晶状体上皮细胞中的几种黏附分子，这可能有助于后囊下混浊的发病机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3617/3386511/b5b31d9ac50a/TOOPHTJ-6-42_F5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3617/3386511/8e05ff14126b/TOOPHTJ-6-42_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3617/3386511/14ae692b6e63/TOOPHTJ-6-42_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3617/3386511/3033700dd0ab/TOOPHTJ-6-42_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3617/3386511/ddc449eb9f69/TOOPHTJ-6-42_F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3617/3386511/b5b31d9ac50a/TOOPHTJ-6-42_F5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3617/3386511/8e05ff14126b/TOOPHTJ-6-42_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3617/3386511/14ae692b6e63/TOOPHTJ-6-42_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3617/3386511/3033700dd0ab/TOOPHTJ-6-42_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3617/3386511/ddc449eb9f69/TOOPHTJ-6-42_F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3617/3386511/b5b31d9ac50a/TOOPHTJ-6-42_F5.jpg

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