去色氨酸1、去亮氨酸12-人迷你胃泌素的α-氘代类似物的合成及核磁共振氢谱研究

Synthesis and 1H-NMR studies of alpha-deuterated analogues of des-Trp1, Nle12-human minigastrin.

作者信息

Amonraksa K, Simonetti M, Da Rin-Fioretto S, Mammi S, Peggion E

机构信息

Department of Organic Chemistry, University of Padua, Italy.

出版信息

Biopolymers. 1990;30(7-8):847-54. doi: 10.1002/bip.360300719.

DOI:10.1002/bip.360300719

Abstract

Three analogues of the tridecapeptide amide H-Leu-(Glu)5-Ala-Tyr-Gly-Nle-Asp-Phe-NH2 were synthetized with alpha-deuterated glutamate residues in specific positions in order to assign unambiguously the 1H nmr spectrum of the parent peptide in water and in water-trifluoroethanol mixtures. The synthetic route is described and the assignment illustrated. A previous, tentative assignment based solely on indirect evidence [Mammi, S., Mammi, N. J. & Peggion, E. (1988) Biochemistry 27, 1374-1379] was partially modified.

摘要

合成了十三肽酰胺H-Leu-(Glu)5-Ala-Tyr-Gly-Nle-Asp-Phe-NH2的三种类似物，其特定位置含有α-氘代谷氨酸残基，以便明确确定母体肽在水中以及水-三氟乙醇混合物中的1H核磁共振谱。描述了合成路线并说明了归属情况。之前仅基于间接证据的初步归属[Mammi, S., Mammi, N. J. & Peggion, E. (1988) Biochemistry 27, 1374-1379]得到了部分修正。

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