Takemoto L, Emmons T, Granstrom D
Division of Biology, Kansas State University, Manhattan 66506.
Curr Eye Res. 1990 Aug;9(8):793-7. doi: 10.3109/02713689008999575.
Polyclonal antisera made against synthetic peptides corresponding to expected tryptic fragments of gamma crystallin have been used to screen tryptic digests of total proteins from cataractous versus normal human lenses. One of these antisera recognizes two peptides that were found in greater amounts from digests of cataractous lenses. These two peptides shared a common sequence that contained an aspartate residue in place of an expected asparagine, suggesting that increased deamidation of this residue had occurred in the human cataractous lens in vivo.
针对与γ-晶状体蛋白预期胰蛋白酶片段相对应的合成肽制备的多克隆抗血清，已用于筛选白内障患者与正常人晶状体总蛋白的胰蛋白酶消化产物。其中一种抗血清识别出两种肽，在白内障晶状体的消化产物中发现这两种肽的含量更高。这两种肽共享一个共同序列，该序列中一个天冬氨酸残基取代了预期的天冬酰胺，表明在人类白内障晶状体中，该残基在体内发生了脱酰胺作用增强。
