Andreassen J, Hoole D, Befus D
Department of Parasitology, University of Copenhagen, Denmark.
Int J Parasitol. 1990 Nov;20(7):905-11. doi: 10.1016/0020-7519(90)90029-m.
Four-day-old and newly excysted H. diminuta were exposed in vitro (37 degrees C; 5% CO2/air atmosphere) to serum and peritoneal cells (1.7-4.4 X 10(5] obtained from rats. Four-day-old worms incubated in serum alone were lysed in titres of less than 16. In assays containing peritoneal cells, leucocytes, predominantly eosinophils and macrophages, adhered to the posterior end of the parasite in serum titre 32, but not in serum titres 64 and 128. In this region of the worms phagocytosis of microtriches by macrophages, microthrix denudation and loss of tegument were noted. Serum-mediated lysis of newly excysted cysticercoids occurred at a serum titre of 64 and leucocyte adherence and phagocytosis of microtriches occurred in serum titres 128 and 256. Attachment of peritoneal cells to worms did not occur in assays containing heat-inactivated serum and it is suggested that regional leucocyte adherence and subsequent parasite damage is complement-mediated.
将4日龄和刚脱囊的微小膜壳绦虫在体外（37摄氏度；5%二氧化碳/空气环境）暴露于从大鼠获取的血清和腹腔细胞（1.7 - 4.4×10⁵）中。仅在血清中孵育的4日龄虫体在效价低于16时会被裂解。在含有腹腔细胞的试验中，白细胞（主要是嗜酸性粒细胞和巨噬细胞）在血清效价为32时附着于寄生虫的后端，但在血清效价为64和128时则不会。在虫体的该区域，观察到巨噬细胞对微毛的吞噬作用、微毛剥脱和皮层损失。新脱囊的囊尾蚴在血清效价为64时发生血清介导的裂解，在血清效价为128和256时发生白细胞对微毛的附着和吞噬作用。在含有热灭活血清的试验中，腹腔细胞不会附着于虫体，提示局部白细胞附着及随后的寄生虫损伤是补体介导的。
