Laboratory for Medical Entomology, Department for Parasitology, Center of Exellence for Toxoplasmosis and Medical Entomology, Institute for Medical Research, University of Belgrade, Dr. Subotića 4, POB 102, 11129, Belgrade, Serbia.
Exp Appl Acarol. 2013 Mar;59(3):367-76. doi: 10.1007/s10493-012-9597-y. Epub 2012 Jul 7.
Past studies in Serbia have reported concurrent infections of Ixodes ricinus ticks with Borrelia burgdorferi sensu lato genospecies, Anaplasma phagocytophilum and Francisella tularensis. As a step forward, this investigation included a broader range of microorganisms and five most common and abundant tick species in Serbia. Five tick species were identified (Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, H. concinna and I. ricinus) and analyzed for the presence of seven pathogens. Anaplasma ovis, A. phagocytophilum, Babesia canis, B. burgdorferi s.l., Coxiella burnetii, Rickettsia helvetica and R. monacensis were detected. Sequencing of samples positive for F. tularensis revealed the presence of Francisella-like endosymbionts. No Bartonella spp. DNA was amplified. Concurrent infections were present in three tick species (D. reticulatus, H. concinna and I. ricinus). The rate of co-infections was highest in I. ricinus (20/27), while this tick species harbored the broadest range of co-infection combinations, with dual, triple and a quadruple infection(s) being detected.
过去在塞尔维亚的研究报告显示，蓖子硬蜱同时感染了伯氏疏螺旋体、嗜吞噬细胞无形体和土拉弗朗西斯菌。作为进一步的研究，本调查包括了更广泛的微生物和塞尔维亚最常见和丰富的五种蜱种。鉴定了五种蜱种（边缘革蜱、血红扇头蜱、璃眼蜱、全沟硬蜱和蓖子硬蜱），并分析了七种病原体的存在情况。检测到绵羊无形体、嗜吞噬细胞无形体、犬巴贝斯虫、伯氏疏螺旋体、柯克斯体、瑞士赫尔尼亚病毒和单形里克次体。对土拉弗朗西斯菌阳性样本进行测序，结果显示存在弗朗西斯菌样内共生体。未扩增到巴尔通体属 DNA。在三种蜱种（血红扇头蜱、全沟硬蜱和蓖子硬蜱）中存在并发感染。在蓖子硬蜱中的共感染率最高（20/27），而这种蜱种携带的共感染组合范围最广，检测到双重、三重和四重感染。
