MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou, China.
Cell Transplant. 2012;21(9):1805-20. doi: 10.3727/096368912X652986. Epub 2012 Jul 5.
Iron oxide nanoparticles (INOPS) are a potential contrast agent for magnetic resonance (MR) tracking of transplanted endothelial cells. The objective of this study was to examine the effect of INOPS labeling on endothelial cells. The mixture of INOPS and poly-l-lysine (PLL) was used to label human endothelial cells. Labeling efficiency was examined by Prussian blue staining, transmission electron microscopy, and atomic absorption spectrometry. The effect of iron oxide concentration on cell viability and proliferation were determined. The correlation of reactive oxygen species (ROS) and apoptosis was also examined. In vitro MRI scanning was carried out using a 1.5T MR system. INOPS-PLL could be readily taken up by endothelial cells and subsequently induce MRI signal intensity changes. However, higher labeling concentration (>50 µg/ml) and longer incubation (48 h) can affect cell viability and proliferation. Mitochondrial damage, apoptosis, and autolysosmes were observed under high INOPS-PLL concentrations, which were correlated to ROS production. INOPS-PLL nanoparticles can be used to label transplanted endothelial cells. However, high concentration of INOPS can impair cell viability, possibly through ROS-mediated apoptosis and autophagy.
氧化铁纳米颗粒（INOPS）是一种潜在的磁共振（MR）示踪移植内皮细胞的对比剂。本研究旨在研究 INOPS 标记对内皮细胞的影响。INOPS 和聚赖氨酸（PLL）的混合物用于标记人内皮细胞。普鲁士蓝染色、透射电子显微镜和原子吸收光谱法检测标记效率。检测了氧化铁浓度对细胞活力和增殖的影响。还研究了活性氧（ROS）和细胞凋亡的相关性。在 1.5T MR 系统上进行了体外 MRI 扫描。INOPS-PLL 可被内皮细胞轻易摄取，并随后诱导 MRI 信号强度变化。然而，较高的标记浓度（>50μg/ml）和较长的孵育时间（48 小时）可能会影响细胞活力和增殖。在高 INOPS-PLL 浓度下观察到线粒体损伤、细胞凋亡和自噬溶酶体，这与 ROS 产生有关。INOPS-PLL 纳米颗粒可用于标记移植的内皮细胞。然而，高浓度的 INOPS 可能会通过 ROS 介导的细胞凋亡和自噬来损害细胞活力。
