Department of Plant Ecophysiology, Institute of Environmental Biology, Utrecht University, Padualaan 8, 3508 CH, Utrecht, The Netherlands.
Photosynth Res. 2012 Sep;113(1-3):207-19. doi: 10.1007/s11120-012-9756-3. Epub 2012 Jul 13.
The effect of temperature and irradiance during growth on photosynthetic traits of two accessions of Arabidopsis thaliana was investigated. Plants were grown at 10 and 22 °C, and at 50 and 300 μmol photons m(-2) s(-1) in a factorial design. As known from other cold-tolerant herbaceous species, growth of Arabidopsis at low temperature resulted in increases in photosynthetic capacity per unit leaf area and chlorophyll. Growth at high irradiance had a similar effect. However, the growth temperature and irradiance showed interacting effects for several capacity-related variables. Temperature effects on the ratio between electron transport capacity and carboxylation capacity were also different in low compared to high irradiance grown Arabidopsis. The carboxylation capacity per unit Rubisco, a measure for the in vivo Rubisco activity, was low in low irradiance grown plants but there was no clear growth temperature effect. The limitation of photosynthesis by the utilization of triose-phosphate in high temperature grown plants was less when grown at low compared to high irradiance. Several of these traits contribute to reduced efficiency of the utilization of resources for photosynthesis of Arabidopsis at low irradiance. The two accessions from contrasting climates showed remarkably similar capabilities of developmental acclimation to the two environmental factors. Hence, no evidence was found for photosynthetic adaptation of the photosynthetic apparatus to specific climatic conditions.
研究了生长过程中温度和光照对拟南芥两个品系光合作用特性的影响。采用因子设计，在 10 和 22°C、50 和 300 μmol 光子 m(-2) s(-1)下培养植物。与其他耐寒草本物种一样，拟南芥在低温下生长会增加单位叶面积的光合能力和叶绿素。在高光强下生长也有类似的效果。然而，几个与能力相关的变量的生长温度和光照表现出相互作用的影响。低温和高光强下生长的拟南芥之间，电子传递能力与羧化能力之间的比例的温度效应也不同。Rubisco 单位活性羧化能力是衡量体内 Rubisco 活性的一个指标，在低光强下生长的植物中Rubisco 单位活性羧化能力较低，但生长温度没有明显的影响。在高温下生长的植物中，三磷酸甘油醛的利用对光合作用的限制较小，当在低光照下生长时，与在高光强下生长相比。这些特性中的几个导致拟南芥在低光照下对光合作用资源利用效率降低。来自不同气候的两个品系对两种环境因素的发育适应能力表现出惊人的相似。因此，没有发现光合作用器对特定气候条件的光合作用适应的证据。
