Kawai T, Okumura K, Hashimoto H, Ito T, Satake T
Second Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Mol Cell Biochem. 1990 Dec 3;99(1):1-8. doi: 10.1007/BF01261387.
The myocardial 1,2-diacylglycerol (DG) and phospholipid levels during ischemia and reperfusion were studied in open-chest dogs by means of sequential epicardial minibiopsies, followed by quantification based on mass measurement technique. 1,2-DG level increased as early as 5 min after coronary ligation but decreased at 30 min. Also as early as 2 min after postischemic (35 min) reperfusion, 1,2-DG level increased transiently compared to pre-reperfusion level. Prazosin inhibited these changes significantly. A significant change in the incidence of reperfusion-induced ventricular tachycardia (VT) was not obtained in the prazosin-treated group. However, the 1,2-DG level 2 min after reperfusion was significantly higher in the ischemic myocardium developed reperfusion-induced VT than in the undeveloped one. Phospholipid levels remained unchanged during ischemia and reperfusion. These results suggest that alpha 1-adrenergic stimulation occurs early in ischemia and reperfusion and leads to 1,2-DG accumulation, which may be involved in the pathogenesis of ischemic and reperfusion injury.
采用连续心外膜微量活检技术，随后基于质量测量技术进行定量分析，对开胸犬缺血和再灌注期间的心肌1,2 - 二酰甘油（DG）和磷脂水平进行了研究。冠状动脉结扎后5分钟，1,2 - DG水平即开始升高，但在30分钟时下降。同样，在缺血（35分钟）后再灌注2分钟时，1,2 - DG水平与再灌注前相比短暂升高。哌唑嗪可显著抑制这些变化。在哌唑嗪治疗组中，再灌注诱导的室性心动过速（VT）发生率未出现显著变化。然而，发生再灌注诱导VT的缺血心肌在再灌注2分钟后的1,2 - DG水平显著高于未发生的缺血心肌。缺血和再灌注期间磷脂水平保持不变。这些结果表明，α1 - 肾上腺素能刺激在缺血和再灌注早期发生，并导致1,2 - DG积累，这可能参与了缺血和再灌注损伤的发病机制。
