印度传统民间医学中使用的星宿菜叶和三白草根的抗生育活性初步研究。

Preliminary study on antifertility activity of Enicostemma axillare leaves and Urena lobata root used in Indian traditional folk medicine.

机构信息

Department of Pharmaceutics, Kakatiya Institute of Pharmaceutical Sciences, Pembarthi (V), Hasanparthy (M), Warangal, Andhra Pradesh, India-506 371.

出版信息

Asian Pac J Trop Med. 2012 Aug;5(8):616-22. doi: 10.1016/S1995-7645(12)60127-8.

DOI:10.1016/S1995-7645(12)60127-8

PMID:22840449

Abstract

OBJECTIVE

To evaluate the possible antifertility activity of Enicostemma axillare (E. axillare) leaves and Urena lobata (U. lobata) root in adult male Wistar albino rats.

METHODS

Six groups of rats were treated with ethanolic (70%v/v) extracts of E. axillare (375 and 750 mg/kg body weight) and U. lobata root (300 and 600 mg/kg body weight) once daily for 55 days. Control groups received the distilled water and vehicle. All the treated rats had corresponding recovery groups. At the end of each treatment periods, animals were killed and organ weights, sperm characteristics, testicular and epididymal biochemicals as well as testicular enzymes were assessed.

RESULTS

The E. axillare and U. lobata at tested doses did not decrease body weight, whereas the weight of testes, epididymides and seminal vesicles were significantly (P<0.01) reduced. Significantly (P<0.01) more reductions in the sperm motility, viability and counts, epididymal and testicular protein contents were noted in the rats treated with higher dose of both the plants. Both the plants at the higher dose caused a marked increase (P<0.01) in sperm morphological abnormalities, testicular cholesterol and ascorbic acid contents were remarkably increased (P<0.01), while, the activities of testicular glucose-6-phosphate dehydrogenase (G-6-PDH) and Δ(5)-3β-hydroxy steroid dehydrogenase (Δ(5)-3β-HSD) were significantly reduced (P<0.01). However, reversal of these changes occurred after 55 days of treatment withdrawal.

CONCLUSIONS

This study suggests that the E. axillare leaves and U. lobata root reversibly inhibited spermatogenesis and steroidogenesis indicating reversible antifertility activity which could partially support the traditional of these plants as male contraceptives.

摘要

目的

评估恩克斯特马（Enicostemma axillare，E. axillare）叶和假地豆（Urena lobata，U. lobata）根在成年雄性 Wistar 白化大鼠中的潜在抗生育活性。

方法

六组大鼠每天用 E. axillare（375 和 750 mg/kg 体重）和 U. lobata 根（300 和 600 mg/kg 体重）的乙醇（70%v/v）提取物处理一次，共 55 天。对照组给予蒸馏水和载体。所有处理过的大鼠都有相应的恢复期大鼠。在每个治疗期结束时，处死动物，评估器官重量、精子特征、睾丸和附睾生化指标以及睾丸酶。

结果

E. axillare 和 U. lobata 在测试剂量下不会降低体重，而睾丸、附睾和精囊的重量则显著（P<0.01）降低。用两种植物的高剂量处理的大鼠，精子活力、活力和计数、附睾和睾丸蛋白含量显著（P<0.01）降低。两种植物在高剂量下都导致精子形态异常显著增加（P<0.01），睾丸胆固醇和抗坏血酸含量明显增加（P<0.01），而睾丸葡萄糖-6-磷酸脱氢酶（G-6-PDH）和 Δ(5)-3β-羟甾醇脱氢酶（Δ(5)-3β-HSD）的活性显著降低（P<0.01）。然而，这些变化在治疗停止 55 天后得到逆转。