改良的从土壤中提取细菌 mRNA 的方法。
Improved protocol for the extraction of bacterial mRNA from soils.
机构信息
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.
出版信息
J Microbiol Methods. 2012 Oct;91(1):62-4. doi: 10.1016/j.mimet.2012.07.016. Epub 2012 Jul 27.
PMID:22841738
Abstract
An improved protocol for extraction of prokaryotic mRNA from soil samples was developed by modifying the extraction procedure to obtain higher yields of mRNA and to reduce co-extraction of humic acids. The modified protocol was found to be more robust and efficient compared to the original protocol by Griffiths et al. (2000) without compromising with the quality and quantity of RNA.
摘要
本研究通过改良提取程序，提高了土壤样品中原核生物 mRNA 的提取产量，减少了腐殖酸的共提，从而建立了一种改良的土壤样品中原核生物 mRNA 提取方法。与 Griffiths 等人（2000 年）建立的原始方法相比，该改良方法更为稳健、高效，同时不会影响 RNA 的质量和数量。
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