Gavrilova L O, Autenshlius A I
Pediatriia. 1990(10):33-8.
The nucleolar test was used to study sensitization of peripheral blood lymphocytes from the newborn and infants. The test is based on the counting of cells with a clarification area around nucleoli after incubation with antigens and microbial bodies of the pathogenic and opportunistic microflora. It has been established that the peripheral blood from children with infectious-inflammatory diseases and from those vaccinated with BCG shows an increase in the sensitized lymphocyte count which is influenced by child's trophicity at birth and by administration of antibacterial therapy. The use of the nucleolar test as a diagnostic aid is advisable to identify the disease etiology.
核仁试验用于研究新生儿和婴儿外周血淋巴细胞的致敏作用。该试验基于在与致病性和机会性微生物群的抗原及菌体孵育后,对核仁周围有清亮区的细胞进行计数。已证实,患有感染性炎症疾病的儿童以及接种卡介苗的儿童的外周血中,致敏淋巴细胞计数会增加,这受到儿童出生时的营养状况和抗菌治疗的影响。建议使用核仁试验作为诊断辅助手段来确定疾病病因。