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[利用FP品牌材料从水中浓缩脊髓灰质炎病毒]

[Concentration of the poliomyelitis virus from water on FP-brand material].

作者信息

Aĭzen M S, Kazantseva V A, Drozdov S G, Sadovskiĭ B F, Bazarov A P

出版信息

Vopr Virusol. 1979 Sep-Oct(5):554-8.

PMID:228498
Abstract

A method of filtration through FP cloth (Petryanov's cloth) has been suggested for concentration of small virus quantities from large volumes of water. The effectiveness of virus removal from water of different degrees of contamination at virus concentrations of 0.05--0.1--0.5--2.5--12.5 PFU/ml was studied. About 88--100% of the initial virus content was removed from sewage, 80--90% from river water, 10--90% from tap water. When the effect of the eluting solutions for the removal of the virus retained on the filter was compared, 1 M NaCl solution in glycocol buffer, pH 11.5, was found to be the most effective. The procedure is characterized by a high rate of filtration, low labour consumption, and simple equipment for filtration.

摘要

有人提出一种通过FP布(彼得里亚诺夫布)过滤的方法,用于从大量水中浓缩少量病毒。研究了在病毒浓度为0.05--0.1--0.5--2.5--12.5 PFU/ml时,从不同污染程度的水中去除病毒的效果。污水中约88--100%的初始病毒含量被去除,河水中为80--90%,自来水中为10--90%。比较用于去除保留在过滤器上的病毒的洗脱液的效果时,发现pH为11.5的甘氨酸缓冲液中的1 M NaCl溶液最有效。该方法的特点是过滤速度快、劳动力消耗低且过滤设备简单。

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